AIM:To discuss the effects of tobramycin-dexamethasone(TobraDex) eye drops combined with aciclovir eye drops (ACV) to treat herpes simplex keratitis (HSK) .METHODS: Forty-three cases 46 eyes with HSK were divided into two groups randomly, one group of TobraDex eye drops was with 23 cases 25 eyes,another group of recombinant bovine fibroblast growth factor eye drops was with 20 case 21 eyes, clinical effect was analyzed.RESULTS: The shallow layer was cured completely, the effectiveness of in-depth TobraDex was 87.5%, and that of the group of recombinant bovine fibroblast growth factor was 60.0%, the average cure time of the TobraDex group was 12 days,and recombinant bovine fibroblast growth factor group was 17 days. CONCLUSION: The TobraDex eye drops combined with ACV eye drops to treat HSK can enhance the curative effect, shorten the course and the recrudescence,especially in in-depth HSK.