AIM:To evaluate the effect of sclera and amniotic membrane preserved by long-term cryopreservation with glycerol for treating the exposure of hydroxyapatite artificial eye stock. METHODS:Thirteen patients 13 eyes with exposure of hydroxyapatite artificial eye stock were treated by debridement and transplantation with sclera and amniotic membrane preserved by long-term cryopreservation with glycerol. The follow-up was 6 months to 1 year to observe the time of vascularization of sclera and epithelization of amniotic membrane.RESULTS: Thirteen patients 13 eyes were healed well, the time of vasculariztion of sclera was 10 days after operation and the time of epithelization of amniotic membrane was 7 days after operation. No conjunatival sac stenosis or re-exposure of artificial eye stock was observed. CONCLUSION: Sclera and amniotic membrane preserved by long-term cryopreservation with glycerol can be used to patch up the exposure of hydroxyapatite artificial eye stock. It is an effective method with high successful rate.