AIM:To investigate clinical effect of DV-100 VEP visual exam and therapeutic system in treating amblyopia.METHODS:DV-100 VEP visual exam and therapeutic system were used to test PVEP of amblyopia of 210 cases 346 eyes,the most suitable visual stimulating signal of amblyopia eyes was measured,and vision training software was made by which amblyopia vision therapy was done at home.RESULTS:All of cases were followed up for 24-26 months,efficient rate of treatment was 90.5%.With the improvement of visual acuity,PVEP P100 time was shorter and amplitude value was increased.Treating result of young age groups was better than that of older age group.CONCLUSION:Personal amblyopia vision training software can be made by using VEP visual exam and therapeutic system,and the outcome of therapy can be monitored by PVEP.