·AIM:To compare the inhibitory effect of tetrandrine(Tet)on fibroblast strains respectively from normal conjunctival and pterygial specimens and provide a new thought in evaluating effect of a drug.METHODS :Different concentrations of Tet solution were added to the third passage normal human conjunctival flbroblasts(HCF)and human pterygium fibroblasts(HPF).After 1,3 days in culture,the number of cells was measured by 3-(4,5-dimethyl-2thiazol)-2,5-diphenyl-2H-tetrazolium bromid(MTT).RESULTS:At 1 day,there was no statistical difference in the number of HPF and HCF in 4×10-5mol/L and 2×10-5mol/L Tet tested groups(P>0.05).The number of HCF was larger than that of HPF in other Tet tested groups(P<0.01).At 3 day,the number of HPF was larger than that of HCF in 4×10-5mol/L Tet tested group(P<0.01).There was no statistical difference in the number of HPF and HCF in other Tet tested groups(P>0.05).CONCLUSION:In view of the increased proliferation of HPF than that of HCF,2×10-5mol/L Tet and other lower-dose Tet has damage to the normal HCF when they exhibit significant antiproliferative effect on cultured HPF at 3 day.