AIM:To obtain the inhibititive effects of spironolactone(SL)on corneal neovascularization(CRNV)induced by alkali burn in rats.METHODS:Totally 42 SD rats were divided into three groups randomly,6 rats were in normal group;CRNV models induced by alkali burn were established on the other 36 SD rats,which were randomly divided into study group and control group,18 in each group.Study group was treated orally with SL(100mg/kg)and control group was treated with the same volume of normal saline,once a day by gastric intubation.The area of CRNV was observed and calculated by slit-lamp microscope at the 4th,7th and 14th day.RESULTS:The rats of control group appeared new vascular buds on 4th day,its growth velocity was quick and became a net on the 14 day.The CRNV of study group was smaller and its growth velocity was slow.Compared with control group,the CRNV area of study group was significant smaller(P<0.05).CONCLUSION:SL can inhibit the occurrence of CRNV.