AIM:To investigate the inhibitory effects of berbamine on fibroblasts in Tenon’s capsule and filtering channel of rabbits.METHODS: Rabbit Tenon’s capsule fibroblasts were treated with berbamine at different concentrations (0-100mg/L) for different hours. CCK8 assay was used to determine proliferation inhibition of rabbit Tenon’s capsule fibroblasts. Flow cytometry was performed to examine cell cycle profile and cell apoptosis rate in response to berbamine treatment. Conjunctive and sclera of filtering area of the rabbits suffered trabeculectomy were examined by pathologic histology examination.RESULTS: Berbamine inhibited the proliferation of rabbit Tenon’s capsule fibroblasts in a dose-and time-dependent manner. Flow cytometric analysis revealed that Tenon’s capsule fibroblasts treated by berbamine were arrested at G1 phase and apoptotic cells increased from 0.64% to 31.86% after treatment with berbamine at 20mg/L for 9 hours (P<0.01). HE stain showed fibroblasts in filtering channel of rabbit were inhibited significantly by berbamine.CONCLUSION: Berbamine could inhibit the growth of fibroblasts in vitro and vivo in rabbit Tenon’s capsule and filtering channel. The induction of cell apoptosis might be one of the mechanisms for suppressing the growth of fibroblasts.