AIM:To discuss morphological character of central serous chorioretinopathy (CSC) under 3D-optical coherence tomography(OCT) and correlation with visual acuity. METHODS:Totally 46 patients(50 eyes)were checked with 3D-OCT, heights,ranges and volumes of nerve epithelial layer detachment in macular areas were measured,and correlation analysis of statistical software SPSS 13.0 was used to calculate correlation between the results above and visual acuity. RESULTS:There was a negative correlation between height of detachment in macular areas and visual acuity(r=-0.78,P=0.00),but no correlation between ranges,volumes and visual acuity (r=-0.39,P=0.26；r=-0.09,P=0.80). CONCLUSION:Heights of detachment in macular areas were the main factor that influenced visual acuity,high resolution 3D-OCT can get images approaching to histopathology of ophthalmology in CSC affected eyes,so as to get clinical implications in analyzing diagnosis,differential diagnosis,degrees of disease and was most valuable in supervision after the therapy.