AIM:To investigate the efficacy of multilayer amniotic membrane cover for traumatic corneal perforation and provide a new solution for patients treatment. METHODS:Totally 20 cases (20 eyes) with traumatic corneal perforation were treated with multilayer amniotic membrane cover for corneal perforation. Follow-up ranged 3-6 months. The formation of anterior chamber, duration of the amniotic membrane and visual acuity changes were observed. RESULTS:The anterior chamber reformed at the first postoperative day in all cases. The amniotic membrane covering the surface of cornea began to dissolve one week after the surgery. The epithelialization was observed one mouth after the surgery, perforation area healed with scar tissue. The vision was not improved in all patients after operation. CONCLUSION:Multilayer amniotic membrane cover is an effective and safety method for traumatic corneal perforation. It can maintain the eyeball structure complete, relieve the inflammation and provide good condition for further penetrating keratoplasty.