To observe the effect of CD44-specific antisense oligonucleotide on extracellular matrix（ECM） synthesis of human trabecular meshwork cells, then to discuss the possible relationship between CD44 and primary open angle glaucoma（POAG）. METHODS: CD44-specific antisense oligonucleotide were delivered with cationic lipid to cultured human trabecular meshwork cells. Effect of CD44-specific antisense oligonucleotide on synthesis of collagen-Ⅰ, Laminin were examined by immunohistochemistry and the synthesis of hyaluronic acid were examined by radioimmunoassay. RESULTS: Three different concentrations of CD44 antisense oligonucleotide could promote the synthesis of collagen-Ⅰ, Laminin in trabecular meshwork cells and in a concentration-dependent manner. But CD44 antisense oligonucleotide inhibited the synthesis of hyaluronic acid. CONCLUSION: CD44 antisense oligonucleotide promotes the synthesis of collagen-Ⅰand Laminin but inhibits the synthesis of hyaluronic acid. CD44 may play a role in pathogenesis of POAG by affecting the synthesis of ECM.