To observe the fibroblasts of normal bulbar conjunctival and conjunctivochalasis cultivated by different medium as well as qijingmingmu soup serum in order to screening the appreciate method for the cultivation of fibroblasts of conjunctivochalasis. METHODS: We collected loose conjuctiva tissue from 16 cases of conjunctivochalasis patients, pterygium tissue from 11 cases, and normal conjuctiva tissue from 5 cases. The growth state of fibroblast was observed to select the most suitable medium for the growth of fibroblasts of conjunctivochalasis. RESULTS: DMEM/F12 can make a few fibroblast of conjunctivochalasis overflow from the tissue, but can not subculture. DMEM-H(contaied 100mL/L FBS, 1μL/mL FGS) can make the fibroblast of conjunctivochalasis grow smoothly, and can transfer. CONCLUSION: The most appropriate training medium for the fibroblast of conjunctivochalasis is DMEM-H, which adds 100mL/L fetal bovine serum, 1μL/mL FGS, 100U/mL penicillin, and 100μg/mL streptomycin.