AIM: To evaluate the effectiveness of treating ocular surface disorders using 100% autologous serum eye drops. METHODS: Totally 35 cases (35 eyes) who had persistent corneal epithelial defects or ulcer that were nonresponsive to conventional medical treatment or amniotic membrane transplantation or lamellar keratoplasty were reviewed.These patients were treated with 100% autologous serum eye drops for 2-6 weeks. Its symptoms and corneal epithelial repair were observed. RESULTS:In 35 eyes ,12 eyes (34.3%) healed within 2 weeks,14 eyes (40.0%) healed within 4weeks.But the corneal epithelium of 9 eyes (25.7%)did not recover within 4 weeks. In herpetic keratitis and neurotrophic keratopathy the cure rate and improvement rate were 47.6% respectively, 95.2% healed within 4 weeks by using 100% autologous serum eye drops.No side effects and secondary infections were detected. CONCLUSION: The use of 100% autologous serum eye drops appears to be an effective and safe medical therapy in the treatment of persistent corneal epithelial defects or ulcer especially caused by the herpetic keratitis and neurotrophic keratopathy.