Blood oxygen level dependence-functional magnetic resonance imaging(BOLD-fMRI)utilizing the endogenous paramagnetic material-deoxygenated hemoglobin as a contrast agent gains signal changes of MRI in cortical intravascular and obtains images of the active area. Due to accurate location, intuitive and noninvasive, BOLD-fMRI is applied widely and provides objective evidence for amblyopia neurogenesis mechanism, ocular dominance column, motion perception and color vision.