AIM:To explore the differences of expression pattern of microRNA(miRNA)in plasma from Vogt-Koyanagi-Harada(VKH)syndrome patients and normal controls, searching for diseases-relating biomarkers.

METHODS:Blood samples from 10 cases of VKH patients and 10 cases of normal control were collected to extracted total RNA in plasma. The cDNA was synthesized by reverse transcription, and then underwent real time PCR in a 96 well plate from miRCURY LNA^TM Universal RT microRNA PCR panels.Original Ct value was obtained. The Exiqon GenEx qPCR analysis software was used to analyse the original data and the differential miRNAs was obtained and analyzed by bioinformatics.

RESULTS:The panels contained 179 miRNAs classes from human individuals. In comparison with the healthy controls,there were 20 differential miRNAs, in which 12 miRNAs were up regulated and 8 miRNA were down regulated(all P<0.05). These microRNAs participated in the regulation of various signaling pathways.

CONCLUSION:The VKH patients and healthy controls have significantly different expression profiles of microRNA.The differential expression of microRNA may be involved in the pathogenesis of VKH.