AIM: To evaluate the regulatory effect of microRNA-132(miR-132)in human umbilical vein endothelial cell(HUVEC).

METHODS: In vitro cultured human umbilical vein endothelia cells in hypoxic environment for 6h, then maintained under normal oxygen condition for 3h, 6h, 12h, 24h. miR-132 and peroxisome-proliferator-activated receptor-γ coactivator-1α(PGC-1α)expression was detected by quantitative Real-time polymerase chain reaction and Western blot analysis. Human umbilical vein endothelial cells transfected miR-132 mimic and miR-132 inhibitor(anti-miR-132)were measured by quantitative Real-time polymerase chain reaction and Western blot.

RESULTS: miR-132 and PGC-1α expression was significantly(P<0.01)upregulated in the hypoxic environment of cells at 3h compared with the normal oxygen condition. After cells transfection, the hypoxic environment the miR-132 and PGC-1α expression were markedly increased compared with the normal oxygen condition. The cells transfected miR-132-mimic, the expression of the miR-132 and PGC-1α were higher than that of transfected anti-miR-132 and contrast group(P<0.01).

CONCLUSION: miR-132 level is highly expressed in the HUVEC under hypoxia and may be an effect of regulation for PGC-1α.