AIM: To study the infiltration of polymorphonuclear neutrophils(PMNs)after conjunctival flap covering in alkali-burned cornea.

METHODS: Rabbit cornea alkali-burned model was made, then 50 rabbits were randomly divided into the experimental group(n=25)and the control group(n=25). At the same time the surgery of conjunctival flap covering was given to rabbits of the experimental group. The condition developing of alkali-burned cornea was observed by slit lamp biomicroscopy, and took photos in two groups. The infiltration of PMNs was identified by hematoxylin eosin(HE)staining in different periods.

RESULTS:The quantity of PMNs increased on the 3d, reached the lower level on 7d, shown a peak on the 14d, then decreased gradually. PMNs level of the experimental group was significantly lower than that in the control group, and the difference of 3, 14 and 21d was significant(P<0.05).

CONCLUSION: During the wound healing process, alkali-burned cornea has close relation with the infiltration of PMNs. The treatment of conjunctival flap covering for the severe alkali-burned cornea was found to have good effect.