AIM: To observe the accuracy of the experimental choroid neovascularization(CNV)changes evaluated by CD105 immunohistochemical examination.

METHODS: Twenty-four male Brown Norway(BN)rats were randomly divided into the control group(6 rats)and the experimental group(18 rats). The CNV were induced by 659nm krypton laser. And the power was 360mW, exposure time was 0.05s, spot diameter was 50μm. The formation rate of CNV and the average optical density(AOD)of leakage flare were observed by fluorescein fundus angiography(FFA)and indocyanine green angiography(ICGA)on the seventh day, the fourteenth day and the twenty-first day after laser photocoagulation. The histopathology changes of CNV and the AOD of CD105 were observed by eyeball exemplar.

RESULTS: The CNV appeared on the seventh day after laser photocoagulation, and reached the peak on the fourteenth day and twenty-first day after laser photocoagulation. The formation rate of CNV were 77.08%, 85.42%, 89.58%, at 7, 14 and 21d after laser photocoagulation. From 7 to 21d after laser photocoagulation, the AOD of leakage flare increased gradually(P<0.05). There had significant differences between 7 days' outcome and 14 days' outcome(P<0.05), and had no significant differences between 14 days' outcome and 21 days' outcome(P>0.05). From 7 to 21d after laser photocoagulation, the expression of CD105 increased gradually, and then decreased gradually, and from 14 to 21d, there had significant differences between 7 days' outcome and 14 days' outcome of AOD(P<0.05), and had no significant differences between 14 days' outcome and 21 days' outcome(P>0.05).

CONCLUSION: Immunohistochemical outcomes of CD105 are highly consistent with fundus angiography outcomes of CNV changing regularity.