Corneal endothelial cell(CEC)is the most critical part for the cornea, of which activity can influence the postoperative vision. It is very important for the clinical cornea preservation considering the function and its self-purification of donor cornea. There are a variety of classical methods, which can significantly prolong the saving time of donor cornea with its good quality of CEC. We reviewed the published papers about present preservation methods of cornea, which can give us many suggestions for the clinical cornea preservation.