Anti-fibrotic effect of rosmarinic acid on inhibition of pterygium epithelial cells
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Yu-Wen Hsu. Department of Optometry, Da-Yeh University, No.168, University Rd., Dacun, Changhua 51591, Taiwan, China.; Fung-Jou Lu. Institute of Medicine, College of Medicine, Chung Shan Medical University, No.110, Sec.1, Jianguo N. Rd., Taichung City 40201, Taiwan, China.


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Supported by Ministry of Science and Technology, Taiwan (No.NSC 106-2314-B-212-004).

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    AIM: To investigate the anti-fibrosis effect of rosmarinic acid (RA) in pterygium epithelial cells (PECs) to determine if RA is a potent agent for treating pterygium. METHODS: The PECs (1×104 cells/mL) were treated with 100 μmol/L of RA for 1, 3 and 6h. After RA treatment, the cell viability was determined by staining with acridine orange/DAPI and analysis via a NucleoCounter NC-3000. The protein expression levels of type I collagen, transforming growth factor beta-1 (TGF-β1), TGF-β type II receptor (TGF-βRII), p-Smad1/5, p-Smad2, p-Smad3, and Smad4 of the cell lysates were measured by Western blot analysis. RESULTS: The cell viability of PECs was significantly decreased after RA treatment (P<0.01). As the result, RA reduced the protein expression of type I collagen and TGF-β1 of PECs. Additionally, RA also inhibited TGF-β1/Smad signaling by decreasing the protein expressions of TGF-βRII, p-Smad1/5, p-Smad2, p-Smad3, and Smad4. CONCLUSION: This study demonstrate that RA could inhibit fibrosis of PECs by down-regulating type I collagen expression and TGF-β1/Smad signaling. Therefore, RA is a potent therapeutic agent for the treatment of pterygium.

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Ya-Yu Chen, Chia-Fang Tsai, Ming-Chu Tsai, et al. Anti-fibrotic effect of rosmarinic acid on inhibition of pterygium epithelial cells. Int J Ophthalmol, 2018,11(2):189-195

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  • Received:June 23,2017
  • Revised:November 20,2017
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  • Online: February 06,2018
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