Citation:Yao XL,Peng QH,Peng J,Tan HY,Wu QL,Wu DL,Chen M,Li CK,Li D,Zhu HA.Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye.Int J Ophthalmol 2010;3(2):114-119,doi:10.3980/j.issn.2222-3959.2010.02.05
Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye
  
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DOI:10.3980/j.issn.2222-3959.2010.02.05
Key Words:dry eye; lacrimal gland; extract of buddleja officinalis; androgen
Fund Project:National Natural Science Foundation of China (No.30772824); Special Fund from Doctoral Program in Colleges and Universities Affiliated to Ministry of Education, China(No.200805410004); Natural Science Fund of Hunan Province of China(No.07JJ3049); Science & Technology Department of Hunan Province fund of China(No.2009FJ3001); Graduate student's innovative fund of Hunan Province of 2008, China(2008 No.68)
                             
AuthorInstitution
Xiao-Lei Yao Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha , Hunan Province, China
Qing-Hua Peng Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha , Hunan Province, China
Jun Peng Medical School of Nanhua University, Hengyang, Hunan Province, China
Han-Yu Tan Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha , Hunan Province, China
Quan-Long Wu Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha , Hunan Province, China
Da-Li Wu Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha , Hunan Province, China
Mei Chen Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha , Hunan Province, China
Chuan-Ke Li Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha , Hunan Province, China
Dian Li Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha , Hunan Province, China
Hui-An Zhu Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha , Hunan Province, China
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Abstract:
      AIM: To assess the effects of extract of Buddleja officinalis on tear secretion volume, tear film stability, expressions of TGF-β1, IL-1β, TNF-α in lacrimal gland of castrated rabbits with dry eye.

    METHODS: A total of 30 victory rabbits were divided averagely into normal group(A), model group(B), therapy group with low dose extract of Buddleja officinalis (C), therapy group with high dose extract of Buddleja officinalis (D) and therapy group with genistein (E). The dry eye model was established with orchiectomy on Group B, C, D, E. Group C, D, E were administered intragastrically with corresponding dose extract of Buddleja officinalis or genistein for 30 days. All rabbits were detected with SIT. TGF-β1, IL-1β, TNF-α were detected with immunohistochemistry and the ultrastructure of lacrimal gland was observed under transmission electron microscope.

    RESULTS: The SIT value of group C, D, E were respectively 13.167±4.957, 14.667±5.279, 8.667±0.516, obviously higher than that of group B 5.667±2.338 (P<0.01). The positive expression of IL-1β in acinar cell and glandular tube cell of group C, D were 0.470±0.048, 0.510±0.088, obviously lower than that of group B 0.770±0.118 (P<0.01). The positive expression of TNF-α of group C, D were 0.498±0.156, 0.435±0.069, obviously lower than that of group B 0.769±0.095 too (P<0.01). The positive expression of TGF-β1 of group C, D were 0.406±0.171, 0.497±0.147, obviously higher than that of group B 0.222±0.113(P<0.01). Any result of group C, D was positive compared with that of group E (P <0.05). Ultrastructure of the lacrimal gland of group C, D, E was well preserved, especially in D group it was remarkable.

    CONCLUSION: The extract of Buddleja officinalis can adjust lacrimal gland partial inflammation of dry eye.

    

PMC FullText Html:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3340767/
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