AIM: To study the tear film stability after lamellar keratoplasty. METHODS: Five female and eight male patients with lamellar keratoconus, aged from 18 to 32, were involved. After lamellar keratoplasty, SchirmerⅠtest(SⅠt), tear break-up time(BUT) test, fluorescein staining test were used to judge the effect of the surgery at different time point. RESULTS: The SⅠt were greatly increased in 7 days post operation (11.86±2.28 -25.14±1.97, 19.86±1.61) (P<0.05), there is no significant difference between 2nd month, 3rd month post-operative and pre-operation (11.86±2.28 - 14.57±1.48, 8.14±0.86) (P>0.05). The mean break-up time decreased in 7 days post operation (5.00±1.31 - 2.71±0.18, 2.57±0.20, 2.71±0.36, 2.43±0.20)(P<0.05). The mean scores of fluorescence increased post-operatively (0.14±0.14 - 8.00±0.00, 8.00±0.00, 8.00±0.00, 7.57±0.20) (P <0.01). CONCLUSION: Lamellar keratoplaty influence the tear film stability, artificial tears and improving corneal epithelium cured medicine should be used after surgery.