Citation:Liu GD,Zhang H,Wang L,Han Q,Zhou SF,Liu P.Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells.Int J Ophthalmol 2013;6(3):280-285,doi:10.3980/j.issn.2222-3959.2013.03.05
Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells
Received: September 03, 2012  Revised: April 20, 2013
Email this Article  Add to Favorites  Print
DOI:10.3980/j.issn.2222-3959.2013.03.05
Key Words:hydrogen  Lipopolysaccharides  miR-9  miR-21  miR-199  Toll-like receptor 4
Fund Project:Foundation of Heilongjiang Educational Committee Project, China (No. 12511313); Heilongjiang Provincial Foundation for Youths Project, China (No. QC2011C119); Research Program of Heilongjiang Provincial Health Office, China (No. 2009-197)
AuthorInstitution
Guo-Dan Liu Department of Ophthalmology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
 
Hong Zhang Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China 
Lin Wang Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China 
Qing Han Department of Ophthalmology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
 
Shi-Feng Zhou Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China 
Ping Liu Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China 
Hits: 271
Download times: 248
Abstract:
      AIM:To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities.

    METHODS:Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS-induced signaling proteins, including Myd88, IKK-β, NF-κB, and PDCD4, were detected by Western blotting.

    RESULTS:The results demonstrated a marked down-regulation of miR-9 and miR-21 and up-regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-β was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-κB expression.

    CONCLUSION:The results in the present study indicate that miR-9, miR-199 and miR-21 play an important role in the anti-inflammatory regulation of LPS-activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury.

PMC FullText Html:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693006/
View Fulltext  View/Add Comment  Download reader