Citation:Liu GD,Zhang H,Wang L,Han Q,Zhou SF,Liu P.Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells.Int J Ophthalmol 2013;6(3):280-285,doi:10.3980/j.issn.2222-3959.2013.03.05
Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells
Received:September 03, 2012  Revised:April 20, 2013
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DOI:10.3980/j.issn.2222-3959.2013.03.05
Key Words:hydrogen  Lipopolysaccharides  miR-9  miR-21  miR-199  Toll-like receptor 4
Fund Project:Foundation of Heilongjiang Educational Committee Project, China (No. 12511313); Heilongjiang Provincial Foundation for Youths Project, China (No. QC2011C119); Research Program of Heilongjiang Provincial Health Office, China (No. 2009-197)
                 
AuthorInstitution
Guo-Dan Liu Department of Ophthalmology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin , Heilongjiang Province, China
Hong Zhang Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin , Heilongjiang Province, China
Lin Wang Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin , Heilongjiang Province, China
Qing Han Department of Ophthalmology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin , Heilongjiang Province, China
Shi-Feng Zhou Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin , Heilongjiang Province, China
Ping Liu Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin , Heilongjiang Province, China
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Abstract:
      AIM:To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities.

    METHODS:Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS-induced signaling proteins, including Myd88, IKK-β, NF-κB, and PDCD4, were detected by Western blotting.

    RESULTS:The results demonstrated a marked down-regulation of miR-9 and miR-21 and up-regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-β was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-κB expression.

    CONCLUSION:The results in the present study indicate that miR-9, miR-199 and miR-21 play an important role in the anti-inflammatory regulation of LPS-activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury.

PMC FullText Html:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693006/
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