Citation:?elik F,Ula? F,?zünal ZG,F?rat T,?elebi S,Do?an ?.Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF, TNFα, and caspase 3 levels in an experimental diabetes model.Int J Ophthalmol 2014;7(1):57-61,doi:10.3980/j.issn.2222-3959.2014.01.10
Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF, TNFα, and caspase 3 levels in an experimental diabetes model
Received: April 22, 2013  Revised: August 27, 2013
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DOI:10.3980/j.issn.2222-3959.2014.01.10
Key Words:bevacizumab  caspase 3  experimental diabetes  fasudil  tumor necrosis factor alpha  vascular endothelial growth factor
Fund Project:Supported by the Scientific Research Project Coordination Unit of Abant Izzet Baysal University.
Conflicts of Interest: ?elik F, None; Ula? F, None; ?zünal ZG, None; F?rat T, None; ?elebi S, None; Do?an ü, None.
                 
AuthorInstitution
Fatih ?elik Department of Ophthalmology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey
Fatih Ula? Department of Ophthalmology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey
Zeynep Güne? ?zünal Department of Medical Pharmacology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey
Tülin F?rat Department of Histology and Embryology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey
Serdal ?elebi Department of Ophthalmology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey
?mit Do?an Department of Ophthalmology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey
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Abstract:
      AIM: To evaluate the influence of an intravitreal injection of bevacizumab and fasudil on the retinal vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNFα), and caspase 3 levels in a diabetic rabbit model.

    RESULTS: There was a statistically significant difference in the VEGF and caspase 3 levels between groups (P=0.005 and P =0.013, respectively), but the TNFα level did not differ significantly between groups (P=0.792). It was found that VEGF levels were significantly lower in Group 1 and Group 3 than in Group 2 using the Mann-Whitney U test with the Bonferroni correction (P=0.004 for both comparison). There was no statistically significant difference between other groups with regard to VEGF levels (the P value ranged between 0.015 and 0.886). Although the P values of the caspase 3 levels were 0.015 for Group 1 and Group 4, 0.038 for Group 2 and Group 3, and 0.018 for Group 3 and Group 4, these P values remained above the threshold P value of 0.0083, which was the statistically significant level for post hoc tests.

    CONCLUSION: An intravitreal injection of bevacizumab decreased both the VEGF level, which plays a role in angiogenesis, and the caspase 3 level, which plays a role in apoptosis. Although not as effective as bevacizumab, fasudil had a beneficial effect on the VEGF levels but significantly increased the caspase 3 levels.

PMC FullText Html:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949459/
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