Citation:Kim SJ,Kim H,Park J,Chung I,Kwon HM,Choi WS,Yoo JM.Tonicity response element binding protein associated with neuronal cell death in the experimental diabetic retinopathy.Int J Ophthalmol 2014;7(6):935-940,doi:10.3980/j.issn.2222-3959.2014.06.04
Tonicity response element binding protein associated with neuronal cell death in the experimental diabetic retinopathy
Received:March 18, 2014  Revised:August 15, 2014
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DOI:10.3980/j.issn.2222-3959.2014.06.04
Key Words:aldose reductase  diabetes  tonicity response element binding protein  retinopathy
Fund Project:Supported by the Basic Research Program of the Korea Science & Engineering Foundation (No.2009-0068732); the Basic Research Program of the National Research Foundation of Korea (No.2011-0020163); the Bio-Industry Technology Development Program funded by the Korea Institute of Planning & Evaluation for Technology in Food, Agriculture Forestry & Fisheries (No.112005-3); the BK21 Program and by the MRC program of KRF (R13-2005-012-01001-1).
Conflicts of Interest: Kim SJ, None; Kim H, None; Park J, None; Chung I, None; Kwon HM, None; Choi WS, None; Yoo JM, None.
ACKNOWLEDGEMENTS The abstract of this paper had been presented as e-poster on 2014 ARVO meeting. We would like to thank Ae-Jin Gu for her technical assistance.
                    
AuthorInstitution
Seong-Jae Kim Department of Ophthalmology, School of Medicine, Gyeongsang National University, Jinju 660-751, Korea
;Institute of Health Science, Gyeongsang National University, Jinju 660-751, Korea
Hwajin Kim Department of Anatomy and Neurobiology, BK21 Biomedical Center, School of Medicine, Gyeongsang National University, Jinju 660-751, Korea
;Institute of Health Science, Gyeongsang National University, Jinju 660-751, Korea
Jeongsook Park Department of Anatomy and Neurobiology, BK21 Biomedical Center, School of Medicine, Gyeongsang National University, Jinju 660-751, Korea
;Institute of Health Science, Gyeongsang National University, Jinju 660-751, Korea
Inyoung Chung Department of Ophthalmology, School of Medicine, Gyeongsang National University, Jinju 660-751, Korea
;Institute of Health Science, Gyeongsang National University, Jinju 660-751, Korea
Hyug-Moo Kwon School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute and Science and Technology, Ulsan 689-798, Korea
Wan-Sung Choi Department of Anatomy and Neurobiology, BK21 Biomedical Center, School of Medicine, Gyeongsang National University, Jinju 660-751, Korea
;Institute of Health Science, Gyeongsang National University, Jinju 660-751, Korea
Ji-Myong Yoo Department of Ophthalmology, School of Medicine, Gyeongsang National University, Jinju 660-751, Korea
;Institute of Health Science, Gyeongsang National University, Jinju 660-751, Korea
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Abstract:
      AIM:To studythe contribution of tonicity response element binding protein (TonEBP) in retinal ganglion cell (RGC) death of diabetic retinopathy (DR).

    METHODS:Diabetes was induced in C57BL/6 mice by five consecutive intraperitoneal injections of 55 mg/kg streptozotocin (STZ). Control mice received vehicle (phosphate-buffered saline). All mice were killed 2mo after injections, and the extent of cell death and the protein expression levels of TonEBP and aldose reductase (AR) were examined.

    RESULTS:The TonEBP and AR protein levels and the death of RGC were significantly increased in the retinas of diabetic mice compared with controls 2mo after the induction of diabetes. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL)-positive signals co-localized with TonEBP immunoreactive RGC. These changes were increased in the diabetic retinas compared with controls.

    CONCLUSION:The present data show that AR and TonEBP are upregulated in the DR and TonEBP may contribute to apoptosis of RGC in the DR.

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