Citation:Liu L,Lao W,Ji QS,Yang ZH,Yu GC,Zhong JX.Lycium barbarum polysaccharides protected human retinal pigment epithelial cells against oxidative stress-induced apoptosis.Int J Ophthalmol 2015;8(1):11-16,doi:10.3980/j.issn.2222-3959.2015.01.02
Lycium barbarum polysaccharides protected human retinal pigment epithelial cells against oxidative stress-induced apoptosis
Received:October 06, 2014  Revised:October 27, 2014
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DOI:10.3980/j.issn.2222-3959.2015.01.02
Key Words:lycium barbarum polysaccharides  retinal pigment epithelial cell  apoptosis  age-related macular degeneration
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Lian Liu Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou , Guangdong Province, China
Wei Lao Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou , Guangdong Province, China
Qing-Shan Ji Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou , Guangdong Province, China
Zhi-Hao Yang Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou , Guangdong Province, China
Guo-Cheng Yu Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou , Guangdong Province, China
Jing-Xiang Zhong Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou , Guangdong Province, China
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Abstract:
      AIM: To investigate the protective effect and its mechanism of lycium barbarum polysaccharides (LBP) against oxidative stress-induced apoptosis in human retinal pigment epithelial cells.

    METHODS: ARPE-19 cells, a human retinal pigment epithelial cell lines, were exposed to different concentrations of H2O2 for 24h, then cell viability was measured by Cell Counting Kit-8 (CCK-8) assay to get the properly concentration of H2O2 which can induce half apoptosis of APRE-19. With different concentrations of LBP pretreatment, the ARPE-19 cells were then exposed to appropriate concentration of H2O2, cell apoptosis was detected by flow cytometric analysis. Expression levels of Bcl-2 and Bax were measured by real time quantitative polymerase chain reaction (RT-PCR) technique.

    RSULTS: LBP significantly reduced the H2O2-induced ARPE-19 cells’ apoptosis. LBP inhibited the H2O2-induced down-regulation of Bcl-2 and up-regulation of Bax.

    CONCLUSION: LBP could protect ARPE-19 cells from H2O2-induced apoptosis. The Bcl-2 family had relationship with the protective effects of LBP.

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