Citation:Kong JH,Zheng D,Chen S,Duan HT,Wang YX,Dong M,,Song J.A comparative study on the transplantation of different concentrations of human umbilical mesenchymal cells into diabetic rats.Int J Ophthalmol 2015;8(2):257-262,doi:10.3980/j.issn.2222-3959.2015.02.08
A comparative study on the transplantation of different concentrations of human umbilical mesenchymal cells into diabetic rats
Received:December 29, 2013  Revised:October 10, 2014
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DOI:10.3980/j.issn.2222-3959.2015.02.08
Key Words:diabetic retinopathy; human umbilical mesenchymal stem cells; nerve growth factor; stem cell therapy
Fund Project:Supported by Tianjin Science and Technology Project, China (No.13ZCZDSY01500).
                    
AuthorInstitution
Jia-Hui Kong Clinical College of Ophthalmology, Tianjin Medical University, Tianjin , China
Dan Zheng Clinical College of Ophthalmology, Tianjin Medical University, Tianjin , China
Song Chen 1Clinical College of Ophthalmology, Tianjin Medical University, Tianjin , China 2Tianjin Eye Hospital, Tianjin , China 3Tianjin Institute of Ophthalmology, Tianjin , China 4Tianjin Key Laboratory of Ophthalmology and Visual Sciences, Tianjin , China
Hong-Tao Duan Clinical College of Ophthalmology, Tianjin Medical University, Tianjin , China
Yue-Xin Wang Clinical College of Ophthalmology, Tianjin Medical University, Tianjin , China
Meng Dong, Clinical College of Ophthalmology, Tianjin Medical University, Tianjin , China
Jian Song Clinical College of Ophthalmology, Tianjin Medical University, Tianjin , China
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Abstract:
      AIM: To observe the effects of intravitreal injections of different concentrations of human umbilical mesenchymal stem cells on retinopathy in rats with diabetes mellitus.

    METHODS: Healthy and adult male Sprague-Dawley (SD) rats were randomly assigned to a normal control group (group A), a diabetic retinopathy (DR) blank control group (group B), a high-concentration transplantation group (group C), a low-concentration transplantation group (group D) and a placebo transplantation group (group E). The expression of nerve growth factor (NGF) protein in the retinal layers was detected by immunohistochemical staining at 2, 4, 6 and 8wk.

    RESULTS: The expression of NGF was positive in group A and most positive in the retinal ganglion cell layer. In groups B and E, the expression of NGF was positive 2wk after transplantation and showed an increase in all layers. However, the level of expression had decreased in all layers at 4wk and was significantly reduced at 8wk. In groups C and D, the expression of NGF had increased at 2wk and continued to increase up to 8wk. The level of expression in group C was much higher than that in group D.

    CONCLUSION: DR can be improved by intravitreal injection of human umbilical mesenchymal stem cells. High concentrations of human umbilical mesenchymal stem cells confer a better protective effect on DR than low concentrations.

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