Citation:Zhao HW,Zhao J,Hu LN,Liang JN,Shi YY,Nie C,Qiu CY,Nan XS,Li YX,Gao FL,Liu Y,Dong Y,Luo L.Effect of long-term weightlessness on retina and optic nerve in tail-suspension rats.Int J Ophthalmol 2016;9(6):825-830,doi:10.18240/ijo.2016.06.06
Effect of long-term weightlessness on retina and optic nerve in tail-suspension rats
Received:February 01, 2016  Revised:April 29, 2016
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DOI:10.18240/ijo.2016.06.06
Key Words:weightlessness; retina; optic nerve; tail suspension
Fund Project:Supported by the National Natural Scientific Foundation of China (No. 81271016).
                                      
AuthorInstitution
Hong-Wei Zhao Chinese PLA General Hospital, Beijing , China; Department of Ophthalmology, the 306th Hospital of PLA, Beijing , China
Jun Zhao Department of Ophthalmology, the 306th Hospital of PLA, Beijing , China
Lian-Na Hu Department of Ophthalmology, the 306th Hospital of PLA, Beijing , China
Jing-Nan Liang Institute of Microbiology, Chinese Academy of Science, Beijing , China
Yuan-Yuan Shi Department of Ophthalmology, the 306th Hospital of PLA, Beijing , China
Chuang Nie Department of Ophthalmology, the 306th Hospital of PLA, Beijing , China
Chang-Yu Qiu Department of Ophthalmology, the 306th Hospital of PLA, Beijing , China
Xin-Shuai Nan Anhui Medical University, Hefei , Anhui Province, China
Yu-Xin Li Department of Ophthalmology, the 306th Hospital of PLA, Beijing , China; Third Military Medical University, Chongqing , China
Fu-Lin Gao Department of Ophthalmology, the 306th Hospital of PLA, Beijing , China
Yi Liu Department of Ophthalmology, the 306th Hospital of PLA, Beijing , China
Yu Dong The First hospital of Jilin University, Changchun , Jilin Province, China
Ling Luo Department of Ophthalmology, the 306th Hospital of PLA, Beijing , China
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Abstract:
      AIM: To evaluate the effect of long-term weightlessness on retina and optic nerve in tail-suspension (TS) rats.

    METHODS: A stimulated weightlessness model was established by suspending rats’ tail. After 12wk, the ultrastructure and the number of optic nerve axons were observed by transmission electron microscope. The number of survival retinal ganglion cells (RGCs) was calculated by fluorescent gold retrograde labeling. Retina cells apoptosis was detected by TUNEL staining. The function of optic nerve and retina was evaluated by the visual evoked potential (VEP) and oscillatory potentials (Ops).

    RESULTS: The optic nerve axons were swollen and sparsely aligned, and the lamellar separation and myelin disintegration occurred after 12wk in TS rats. The density of optic nerve axons was 32.23±3.92 (vs 37.43±4.13, P=0.0145), the RGCs density was 1645±46 cells/mm2 (vs 1867±54 cells/mm2 P=0.0000), the incidence rate of retinal cells apoptosis was 5.38%±0.53% (vs 4.75%±0.54%, P=0.0238), the amplitude of VEP-P100 was 15.43±2.14 ?V (vs 17.67±2.17 ?V, P=0.0424), the latency of VEP-P100 was 69.05±5.34ms (vs 62.43±4.87ms P=0.0143) and the sum amplitude of Ops was 81.05±8.34 ?V (vs 91.67±10.21 ?V, P=0.0280) in TS group and the control group, respectively.

    CONCLUSION: Long-term weightlessness can induce the ultrastructural changes and functional depress of the optic nerve, as well as retinal cell damages in TS rats.

PMC FullText Html:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916137/
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