Citation:Wang JW,Liu YM,Zhao XF,Zhang H.Gastrodin protects retinal ganglion cells through inhibiting microglial-mediated neuroinflammation in an acute ocular hypertension model.Int J Ophthalmol 2017;10(10):1483-1489,doi:10.18240/ijo.2017.10.01
Gastrodin protects retinal ganglion cells through inhibiting microglial-mediated neuroinflammation in an acute ocular hypertension model
Received:March 25, 2017  Revised:July 10, 2017
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DOI:10.18240/ijo.2017.10.01
Key Words:gastrodin  retina ganglion cells  microglia  neuroinflammation  acute ocular hypertension
Fund Project:Supported by the Natural Science Foundation of Shandong Province, China (No.ZR2017BH049).
           
AuthorInstitution
Jia-Wei Wang Eye Center of Shandong University, the Second Hospital of Shandong University, Shandong University, Jinan , Shandong Province, China; Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou , Guangdong Province, China
Yao-Ming Liu Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou , Guangdong Province, China
Xiao-Fei Zhao Eye Center of Shandong University, the Second Hospital of Shandong University, Shandong University, Jinan , Shandong Province, China
Han Zhang Eye Center of Shandong University, the Second Hospital of Shandong University, Shandong University, Jinan , Shandong Province, China
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Abstract:
      AIM: To investigate the neuroprotective effect of gastrodin on retinal ganglion cells (RGCs) in an acute ocular hypertension (AOH) rat model and to identify its possible mechanism.

    METHODS: AOH rat model was performed in a randomly selected eye by anterior chamber perfusion and either received an intraperitoneal injection with various concentrations of gastrodin or normal saline. After 2wk, the rats were sacrificed. FluoroGold was used to label survival RGCs. Immunostaining with anti-Iba1 in the retinal flat mounts to calculate the microglia density in the ganglion cell layer (GCL). Changes in microglial cytokines, tumour necrosis factor-alpha (TNF-α) and inducible NO synthase (iNOS) were examined with Western blot and reverse transcription-quantitative polymerase chain reaction. Expression levels of total and phosphorylated p38 mitogen activated protein kinase (MAPK) were determined by Western blot.

    RESULTS: Results showed that AOH induced significant loss of RGCs and severe microglia activation in the GCL. Besides, AOH increased the phosphorylation of p38 MAPK and promoted the release of microglial cytokines in the retinas. Intraperitoneal injection with dose-dependent gastrodin significantly reduced the loss of RGCs and inhibited retinal microglia activation, accompanied with the decreased expression levels of microglial cytokines and p38 MAPK phosphorylation.

    CONCLUSION: Gastrodin exerts a neuroprotective effect on RGCs in an acute glaucoma animal model via inhibiting microglia activation and microglial-mediated neuroinflammation. The finding demonstrates the potential application of gastrodin in the neuroprotective therapy of acute glaucoma and other retinal neurodegenerative diseases characterized by microglia activation and RGCs death.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5638966/
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