Citation:Dong LL,Tang R,Zhai YJ,Malla T,Hu K.DNA vaccine expressing herpes simplex virus 1 glycoprotein C and D protects mice against herpes simplex keratitis.Int J Ophthalmol 2017;10(11):1633-1639,doi:10.18240/ijo.2017.11.01
DNA vaccine expressing herpes simplex virus 1 glycoprotein C and D protects mice against herpes simplex keratitis
Received:April 05, 2017  Revised:September 05, 2017
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DOI:10.18240/ijo.2017.11.01
Key Words:herpes simplex virus 1  keratitis  gC-based DNA vaccine  nanocarrier  immune response
Fund Project:Supported by Natural Science Foundation of Jiangsu Province (No.BK20141346); Nanjing Science and Technology Development Plan (No.201402001).
              
AuthorInstitution
Li-Li Dong Department of Ophthalmology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing University, Nanjing , Jiangsu Province, China; Department of Ophthalmology, Jiangsu Taizhou People’s Hospital, Taizhou , Jiangsu Province, China; Medical School of Southeast University, Nanjing , Jiangsu Province, China
Ru Tang Medical School of Southeast University, Nanjing , Jiangsu Province, China; Department of Ophthalmology, the People’s Hospital of Danyang, Zhenjiang , Jiangsu Province, China
Yu-Jia Zhai Medical School of Southeast University, Nanjing , Jiangsu Province, China
Tejsu Malla Medical School of Southeast University, Nanjing , Jiangsu Province, China
Kai Hu Department of Ophthalmology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing University, Nanjing , Jiangsu Province, China; Nanjing Ning Yi Eye Center, Nanjing , Jiangsu Province, China
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Abstract:
      AIM: To investigate whether DNA vaccine encoding herpes simplex virus 1 (HSV-1) glycoprotein C (gC) and glycoprotein D (gD) will achieve better protective effect against herpes simplex keratitis (HSK) than DNA vaccine encoding gD alone.

    METHODS: DNA vaccine expressing gD or gC combined gD (gD.gC) were constructed and carried by chitosan nanoparticle. The expression of fusion protein gD and gC were detected in DNA/nanoparticle transfected 293T cells by Western-blot. For immunization, mice were inoculated with DNA/nanoparticle for 3 times with 2wk interval, and two weeks after the final immunization, the specific immune responses and clinical degrees of primary HSK were evaluated.

    RESULTS: Fusion protein gD.gC could be expressed successfully in cultured 293T cells. And, pRSC-gC.gD-IL21 DNA/chitosan nanoparticle could effectively elicit strongest humoral and cellular immune response in primary HSK mice evidenced by higher levels of specific neutralizing antibody and sIgA production, enhanced cytotoxicities of splenocytes and nature killer cells (NK), when compared with those of gD alone or mocked vaccine immunized mice. As a result, gC-based vaccine immunized mice showed least HSK disease.

    CONCLUSION: gC-based DNA vaccine could effectively prevent the progress of primary HSK, suggesting that this DNA vaccine could be a promising vaccine for HSK treatment in the future.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686359/
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