Citation:Shentu XC,Ping XY,Cheng YL,Zhang X,Tang YL,Tang XJ.Hydrogen peroxide-induced apoptosis of human lens epithelial cells is inhibited by parthenolide.Int J Ophthalmol 2018;11(1):12-17,doi:10.18240/ijo.2018.01.03
Hydrogen peroxide-induced apoptosis of human lens epithelial cells is inhibited by parthenolide
Received:October 09, 2017  Revised:December 02, 2017
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DOI:10.18240/ijo.2018.01.03
Key Words:parthenolide; apoptosis; human lens epithelial cells; hydrogen peroxide
Fund Project:Supported by the National Natural Science Foundation of China (No.81371000; No.81670834); the Natural Science Foundation of Zhejiang Province (No.LY17H090004); the Zhejiang Traditional Chinese Medicine Project (No.2013ZA080); the Fundamental Research Funds for the Central Universities (No.2017FZA7002).
                 
AuthorInstitution
Xing-Chao Shentu Eye Center, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou , Zhejiang Province, China
Xi-Yuan Ping Eye Center, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou , Zhejiang Province, China
Ya-Lan Cheng Eye Center, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou , Zhejiang Province, China
Xin Zhang Eye Center, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou , Zhejiang Province, China
Ye-Lei Tang The Second Affiliated Hospital of Zhejiang University the School of Medicine, Hangzhou , Zhejiang Province, China
Xia-Jing Tang Eye Center, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou , Zhejiang Province, China
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Abstract:
      AIM: To explore the effect of parthenolide on hydrogen peroxide (H2O2)-induced apoptosis in human lens epithelial (HLE) cells.

    METHODS: The morphology and number of apoptotic HLE cells were assessed using light microscopy and flow cytometry. Cell viability was tested by MTS assay. In addition, the expression of related proteins was measured by Western blot assay.

    RESULTS: Apoptosis of HLE cells was induced by 200 μmol/L H2O2, and the viability of these cells was similar to the half maximal inhibitory concentration (IC50), as examined by MTS assay. In addition, cells were treated with either different concentrations (6.25, 12.5, 25 and 50 μmol/L) of parthenolide along with 200 μmol/L H2O2 or only 50 μmol/L parthenolide or 200 μmol/L H2O2 for 24h. Following treatment with higher concentrations of parthenolide (50 μmol/L), fewer HLE cells underwent H2O2-induced apoptosis, and cell viability was increased. Further, Western blot assay showed that the parthenolide treatment reduced the expression of caspase-3 and caspase-9, which are considered core apoptotic proteins, and decreased the levels of phosphorylated nuclear factor-κB (NF-κB), ERK1/2 [a member of the mitogen-activated protein kinase (MAPK) family], and Akt proteins in HLE cells.

    CONCLUSION: Parthenolide may suppress H2O2-induced apoptosis in HLE cells by interfering with NF-κB, MAPKs, and Akt signaling.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5767651/
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