Protective effects of a composition of Chinese herbs-Gurigumu-13 on retinal ganglion cell apoptosis in DBA/2J glaucoma mouse model
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Zhan-Jun Lu. Department of Ophthalmology, Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao 028000, the Inner Mongolia Autonomous Region, China. Ao Jiang. China-Japan Union Hospital of Jilin University, Changchun, Economic Development Zone, Changchun 130000, Jilin Province, China. jiangao0211@163.com

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Supported by the National Natural Science Foundation of China (No.81660734).

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    Abstract:

    AIM: To explore the concrete mechanism of a Mongolian compound medicine-Gurigumu-13 (GRGM) for glaucoma treatment. METHODS: DBA/2J mice, as glaucoma models, were intragastric administrated with GRGM to study the effect of GRGM on retinal ganglion cells (RGCs). The loss of RGCs was evaluated with the number of RGCs and axons. The expression of the target protein of RGCs or mouse retinas was determined by Western blot. The relative content of malondialdehyde (MDA) was examined by ELISA assay. RESULTS: GRGM distinctly improved retina damage via increasing the number of neurons, RGCs and axons in a concentration dependent manner. Meanwhile, GRGM obviously decreased the high level of MDA and the expression of oxidative stress-related proteins in retinas of DBA/2J mice, but promoted the expression of antioxidant proteins. Additionally, GRGM also significantly inhibited the protein expression of Bip and Chop, which were markers of endoplasmic reticulum stress-induced apoptosis. CONCLUSION: GRGM have obvious protective effects on RGCs in DBA/2J mice, and increase the number of RGCs and axons via inhibiting oxidative stress and endoplasmic reticulum stress.

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Qiu-Li Zhang, Wei Wang, Yan Jiang, et al. Protective effects of a composition of Chinese herbs-Gurigumu-13 on retinal ganglion cell apoptosis in DBA/2J glaucoma mouse model. Int J Ophthalmol, 2018,11(3):363-368

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History
  • Received:August 04,2017
  • Revised:January 15,2018
  • Adopted:
  • Online: March 13,2018
  • Published: