Citation:Xu R,Lin J,Zhao GQ,Li C,Che CY,Xu Q,Liu M.Production of interleukin-1β related to mammalian target of rapamycin/Toll-like receptor 4 signaling pathway during Aspergillus fumigatus infection of the mouse cornea.Int J Ophthalmol 2018;11(5):712-718,doi:10.18240/ijo.2018.05.02
Production of interleukin-1β related to mammalian target of rapamycin/Toll-like receptor 4 signaling pathway during Aspergillus fumigatus infection of the mouse cornea
Received:October 23, 2017  Revised:March 19, 2018
Email this Article  Add to Favorites  Print
DOI:10.18240/ijo.2018.05.02
Key Words:keratitis; interleukin-1β; mammalian target of rapamycin; Toll-like receptor 4; mice
Fund Project:Supported by the National Natural Science Foundation of China (No.81470609; No.81500695).
                    
AuthorInstitution
Rui Xu Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Jing Lin Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Gui-Qiu Zhao Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Cui Li Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Cheng-Ye Che Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Qiang Xu Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Min Liu Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Hits: 247
Download times: 142
Abstract:
      AIM: To elucidate the effect of rapamycin on regulating the production of interleukin (IL)-1β in Aspergillus fumigatus (A. fumigatus)-induced keratitis and to verify whether the expression of IL-1β in A. fumigatus keratitis is associated with the mammalian target of rapamycin (mTOR)/Toll-like receptor 4 (TLR4) signaling pathway.

    METHODS: Fungal keratitis mouse models of susceptible C57BL/6 mice were established using A. fumigatus. The mice were subsequently treated with rapamycin. The protein levels of p-mTOR, TLR4, and IL-1β in normal and infected corneal tissue were measured by Western blot. The TLR4 and IL-1β mRNA levels were determined by real-time polymerase chain reaction (PCR).

    RESULTS: In C57BL/6 mice, rapamycin treatment decreased the clinical scores and production of the pro-inflammatory cytokine, IL-1β. The expression of TLR4, stimulated by A. fumigatus, was reduced as well when the mTOR signaling pathway was suppressed by rapamycin.

    CONCLUSION: Rapamycin is beneficial for the outcome of fungal keratitis and has an inhibitory effect expression of the inflammatory cytokine IL-1β. The inhibitory effect on IL-1β expression can be associated with the mTOR/TLR4 signaling pathway in A. fumigatus infection in mice.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5957020/
PDF Fulltext  Download reader  HTML Fulltext   View/Add Comment