Regulation of lipoxygenase-1 and Dectin-1 on interleukin-10 in mouse Aspergillus fumigatus keratitis
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Gui-Qiu Zhao. Department of Ophthalmology, the Affiliated Hospital of Qingdao University, No.16 of Jiangsu Road, Shinan District, Qingdao 266003, Shandong Province, China. zhaoguiqiu_good@126.com

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Supported by the National Natural Science Foundation of China (No.81470609; No.81300730; No.81500695); China Postdoctoral Science Foundation (No.2018M630482); the National Natural Science Foundation of Shandong (No.ZR2017BH025); Key Research Project of Shandong (No.2018GSF118193); Applied Basic Research Project of Qingdao (No.16-5-1-65-jch).

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    Abstract:

    AIM: To investigate the regulation of lipoxygenase (LOX)-1 and Dectin-1 on interleukin-10 (IL-10) production in mice with Aspergillus fumigatus (A. fumigatus) keratitis. METHODS: The corneas of C57BL/6 mice were pretreated with LOX-1 inhibitor Poly(I) or Dectin-1 siRNA separately before the infection of A. fumigatus. Polymerase chain reaction (PCR) and Western blot were used to detect the expression of IL-10. RESULTS: The mRNA and protein expressions of IL-10 were significantly increased in mice with A. fumigatus keratitis. Compared with the group pretreated with sterile water before infection, Poly(I) pretreatment suppressed IL-10 expression significantly. Compared with the group pretreated with scrambled siRNA before infection, Dectin-1 siRNA pretreatment significantly reduced IL-10 expression in response to A. fumigatus infection. CONCLUSION: LOX-1 and Dectin-1 regulate IL-10 production in mouse A. fumigatus keratitis.

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Cheng-Ye Che, Ke-Lan Yuan, Gui-Qiu Zhao, et al. Regulation of lipoxygenase-1 and Dectin-1 on interleukin-10 in mouse Aspergillus fumigatus keratitis. Int J Ophthalmol, 2018,11(6):905-909

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  • Received:October 18,2017
  • Revised:February 28,2018
  • Adopted:
  • Online: June 12,2018
  • Published: