AIM: To study effects of orthokeratology (Ortho-k) on accommodation function in myopic children. METHODS: A prospective, non-randomized, case-control study was performed from September to October 2016. Eighty-three children with myopia were divided into two groups. One group was treated with the rigid contact lens for overnight Ortho-k, and the other was treated with single-vision spectacle lens (SVL). Accommodation function were assessed by accommodative amplitude (AA), accommodative sensitivity (AS), accommodative lag (Lag), negative relative accommodation (NRA), and positive relative accommodation (PRA) before and 1, 3, 6 and 12mo after treated. RESULTS: Totally 72 myopic children were finished the follow-up: 37 in Ortho-k group and 35 in SVL group. Wearing time had a significant effect on AA, AS, Lag, and NRA of myopic children in two groups (all P<0.05). Meanwhile, there was an interaction effect between wearing time and wearing types (FAA=5.3, FAS=45.5, FLag=7.0, FNRA=3.7, all P<0.05). However, the between-group difference of AA (F=0.1), AS (F=3.2), Lag (F=1.1), NRA (F=0.3), and PRA (F=0.1) showed no significance. AA, AS, NRA, Lag and PRA were improved significantly in Ortho-k group within 1 to 6mo after wearing. CONCLUSION: Wearing Ortho-k can improve the accommodation function of myopic children, which might be one of the mechanisms for myopia control.