Identification of microRNA-mRNA regulatory networks and pathways related to retinoblastoma across human and mouse
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Hui Zhang. Department of Ophthalmology, the Second Hospital of Jilin University, No.218 Ziqiang Street, Nan Guan District, Changchun 130000, Jilin Province, China. zhanghui_m04@163.com

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Supported by the Youth Program of National natural science foundation (2018) of China (No.81802998); 2019 Basic Research Natural Science Foundation (No.20190201150JC); the Norman Bethune Program of Jilin University (No.2015327).

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    Abstract:

    AIM: To explore the mRNA and pathways related to retinoblastoma (RB) genesis and development. METHODS: Microarray datasets GSE29683 (human) and GSE29685 (mouse) were downloaded from NCBI GEO database. Homologous genes between the two species were identified using WGCNA, followed by protein-protein interaction (PPI) network construction and gene enrichment analysis. Disease-related miRNAs and pathways were retrieved from miR2Disease database and Comparative Toxicogenomics Database (CTD), respectively. RESULTS: A total of 352 homologous genes were identified. Two pathways including “cell cycle” and “pathway in cancer” in CTD and enrichment analysis were identified and seven miRNAs (including hsa-miR-373, hsa-miR-34a, hsa-miR-129, hsa-miR-494, hsa-miR-503, hsa-let-7 and hsa-miR-518c) were associated with RB. miRNAs modulate “cell cycle” and “pathway in cancer” pathways via regulating 13 genes (including CCND1, CDC25C, E2F2, CDKN2D and TGFB2). CONCLUSION: These results suggest that these miRNAs play crucial roles in RB genesis through “cell cycle” and “pathway in cancer” pathways by regulating their targets including CCND1, CDC25C, E2F2 and CDKN2D.

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Rui Tian, He Zou, Lu-Fei Wang, et al. Identification of microRNA-mRNA regulatory networks and pathways related to retinoblastoma across human and mouse. Int J Ophthalmol, 2020,13(4):535-544

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History
  • Received:July 20,2019
  • Revised:February 19,2020
  • Adopted:
  • Online: March 03,2020
  • Published: