Citation:Gong Y, Wu GH, Zhang LY, Zhang Z, Liao YH, Liu XT. Effect of nintedanib thermo-sensitive hydrogel on neovascularization in alkali burn rat model. Int J Ophthalmol 2020;13(6):879-885,doi:10.18240/ijo.2020.06.04
Effect of nintedanib thermo-sensitive hydrogel on neovascularization in alkali burn rat model
Received:January 14, 2020  Revised:April 13, 2020
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DOI:10.18240/ijo.2020.06.04
Key Words:nintedanib  alkali burn  neovascularization  cornea  rat
Fund Project:Supported by Zhejiang Province Traditional Chinese Medicine Science and Technology Plan Project (No.2018ZA111); Natural Science Foundation of Zhejiang Province (No.LY19H120001); Zhejiang Provincial Medicine and Health Science and Technology Project (No.2020KY288); Ningbo Natural Science Foundation (No.2019A610353); Ningbo Leading and Outstanding Talents Cultivation Project Selects and Supports Scientific Research Projects (No.NBLJ201801037); Ningbo Public Welfare Science and Technology Plan Project (No.2019C50059); Ningbo Yinzhou District Science and Technology Bureau Agricultural and Social Science and Technology Plan Project (Yanke [2017] No.110).
                 
AuthorInstitution
Yan Gong Department of Ophthalmology, Ningbo Eye Hospital, Ningbo , Zhejiang Province, China
Guo-Hai Wu Department of Ophthalmology, Ningbo Eye Hospital, Ningbo , Zhejiang Province, China
Ling-Yi Zhang Department of Ophthalmology, Ningbo Eye Hospital, Ningbo , Zhejiang Province, China
Zhe Zhang Department of Ophthalmology, Ningbo Eye Hospital, Ningbo , Zhejiang Province, China
Yan-Hong Liao Department of Ophthalmology, Ningbo Eye Hospital, Ningbo , Zhejiang Province, China
Xiao-Tian Liu Department of Ophthalmology, Ningbo Eye Hospital, Ningbo , Zhejiang Province, China
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Abstract:
      AIM: To investigate the effects of nintedanib thermo-sensitive hydrogel (NTH) on neovascularization and related markers in corneal alkali burns of Wistar rats.

    METHODS: NTH was prepared by grinding, and its phase-transition temperature was determined. Thirty specific-pathogen-free Wistar rats served as a model of corneal alkali burn in the right eye were randomly divided into 3 groups (n=10, each): model group treated with 0.9% saline once a day, NTH group with 0.2% nintedanib b.i.d, and dexamethasone group with dexamethasone ointment once a day. The left eye of rats served as the controls. The corneal transparency was observed under a slit-lamp microscope, and the area of neovascularization was calculated. On day 7, the rats were sacrificed, and the cornea was removed and embedded with paraffin, then stained with hematoxylin-eosin, and the expression of vascular endothelial growth factor receptor 2 (VEGFR-2) and CD31 in the corneal tissues of each group was detected by immunofluorescence.

    RESULTS: The phase-transition temperature of nintedanib obtained by grinding was 37℃ after adding artificial tears. The results of the alkali burn model indicated that the growth rate of neovascularization in the NTH group was slower than that in the model group, and the neovascularization area was significantly smaller than that in the model group (P<0.05). Moreover, CD31 and VEGFR-2 expression levels in the NTH group were significantly lower than those in the model group.

    CONCLUSION: NTH becomes colloidal at body temperature, which is beneficial for releasing the drug slowly and can significantly inhibit the neovascularization of corneal induced by alkali burn in rats.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7270255/
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