Citation:Sük?sd AK, Szabadfi K, Szabó-Meleg E, Gáspár B, Stodulka P, Sétáló G Jr, Gábriel R, Nyitrai M, Biró Z, ?brahám H. Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy. Int J Ophthalmol 2020;13(6):927-934,doi:10.18240/ijo.2020.06.11
Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy
Received:June 26, 2019  Revised:March 16, 2020
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DOI:10.18240/ijo.2020.06.11
Key Words:lens epithelial cell  cytoskeleton  capsulotomy  glial fibrillary acidic protein  actin
Fund Project:Supported by EFOP-3.6.3-VEKOP-16-2017- 00009 and GINOP-2.3.2-15-2016-00036 to University of Pécs Medical School; EFOP-3.6.1-16-2016-00004 project; The Higher Education Institutional Excellence Programme of the Ministry for Innovation and Technology in Hungary, within the framework of the 5. thematic programme of the University of Pécs.
                             
AuthorInstitution
Andrea Krisztina Sükosd Department of Ophthalmology, University of Pécs Medical School and Clinical Centre, Pécs 7623, Hungary
Krisztina Szabadfi Department of Experimental Zoology and Neurobiology, University of Pécs, Pécs 7624, Hungary
Edina Szabó-Meleg Department of Biophysics, University of Pécs Medical School and Clinical Centre, Pécs 7624, Hungary; János Szentágothai Research Centre, University of Pécs, Pécs 7624, Hungary
Beáta Gáspár Optimum Laser Centre, Budapest 1124, Hungary
Pavel Stodulka Gemini Eye Clinics, Zlin 76001, Czech Republic
Gyorgy Sétáló Jr János Szentágothai Research Centre, University of Pécs, Pécs 7624, Hungary; Department of Medical Biology and Central Electron Microscopic Laboratory, University of Pécs Medical School, Pécs 7624, Hungary
Róbert Gábriel Department of Experimental Zoology and Neurobiology, University of Pécs, Pécs 7624, Hungary
Miklós Nyitrai Department of Biophysics, University of Pécs Medical School and Clinical Centre, Pécs 7624, Hungary; János Szentágothai Research Centre, University of Pécs, Pécs 7624, Hungary
Zsolt Biró Department of Ophthalmology, University of Pécs Medical School and Clinical Centre, Pécs 7623, Hungary; Optimum Laser Centre, Budapest 1124, Hungary
Hajnalka Abrahám Department of Medical Biology and Central Electron Microscopic Laboratory, University of Pécs Medical School, Pécs 7624, Hungary
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Abstract:
      AIM: To study the effect of mechanical stress on the cytoskeleton in lens epithelial cells following conventional phacoemulsification surgery (CPS) and femtosecond laser-assisted cataract surgery (FLACS).

    METHODS: The cytoskeleton of the epithelial cells of the anterior lens capsules (ALC) removed by CPS and FLACS was examined by immunohistochemistry. Expression of the intermediate filament, glial fibrillary acidic protein (GFAP), and glutamine synthetase (GS) immunoreactivity were detected. In order to map the actin network of cells, fluorescently labeled phalloidin was used. The samples were examined using confocal laser scanning microscopy.

    RESULTS: GFAP expression was visible in a larger number of the epithelial cells after CPS compared to FLACS. In CPS sample’s epithelial cells, GFAP immunoreactivity indicated robust morphological change. Regarding the actin filaments, the presence of tubular elements connecting epithelial cells, regular actin pattern and marked cortical network after CPS were found. Following FLACS, the actin cytoskeleton of the epithelial cells remained densely structured, and the tubular elements were undetectable, however, the above-mentioned regular actin pattern and the marked cortical network were visible.

    CONCLUSION: The conventional removal of the ALC induces more robust changes of the cytoskeleton of the lens epithelial cells.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7270246/
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