Whole transcriptome analysis on blue light-induced eye damage
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Guo-Hui Wang and Xiao-Yun Dong. Key Laboratory of Biological Medicines in Universities of Shandong Province, Weifang Medical University, Weifang 261053, Shandong Province, China. wangguohui1983@163.com; dongxiaoyun377400@wfmc.edu.cn

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Supported by the National Natural Science Foundation of China (No.11802209); the Natural Science Foundation of Shandong Province China (No.ZR2019MA018; No.ZR2019BC095); Shandong Project for Talents Introduction and Development on Youth Innovation Team of Higher Education.

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    Abstract:

    AIM: To analyze abnormal gene expressions of mice eyes exposed to blue light using RNA-seq and analyze the related signaling pathways. METHODS: Kunming mice were divided into an experimental group that was exposed to blue light and a control group that was exposed to natural light. After 14d, the mice were euthanized and their eyeballs were collected. Whole transcriptome analysis was attempted to analyze the gene expression of the eyeballs using RNA-seq to reconstruct genetic networks. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used to reveal the related signaling pathways. RESULTS: The 737 differentially expressed genes were identified, including 430 up and 307 down regulated genes, by calculating the gene FPKM in each sample and conducting differential gene analysis. GO and KEGG pathway enrichment analysis showed that blue light damage may associated with the visual perception, sensory perception of light stimulus, phototransduction, and JAK-STAT signaling pathways. Differential lncRNA, circRNA and miRNA analysis showed that blue light exposure affected pathways for retinal cone cell development and phototransduction, among others. CONCLUSION: Exposure to blue light can cause a certain degree of abnormal gene expression and modulate signaling pathways in the eye.

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Xin-Li Ouyang, Bo-Yu Chen, Yong-Fang Xie, et al. Whole transcriptome analysis on blue light-induced eye damage. Int J Ophthalmol, 2020,13(8):1210-1222

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  • Received:March 30,2020
  • Revised:May 07,2020
  • Adopted:
  • Online: June 22,2020
  • Published: