Citation:Chen WY,Xiao KH,Lin R,Qiu ZR,Chen YM,Lin ZQ,Ke XB,Huang Y.Effect of light-emitting diodes with different color rendering indexes on the ocular tissues of rat.Int J Ophthalmol 2022;15(7):1035-1043,doi:10.18240/ijo.2022.07.01
Effect of light-emitting diodes with different color rendering indexes on the ocular tissues of rat
Received:November 24, 2021  Revised:January 25, 2022
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DOI:10.18240/ijo.2022.07.01
Key Words:light emitting diodes  lacrimal gland  retina  dry eye  color rendering indexes  spectrum
Fund Project:Supported by the Natural Science Foundation of Fujian Province (No.2020J01652); the Undergraduate Innovation and Entrepreneurship Training Program of Fujian Medical University (No.YC2003).
                       
AuthorInstitution
Wen-Yi Chen Department of Ophthalmology and Optometry, Fujian Medical University, Fuzhou , Fujian Province, China
Kun-Hong Xiao Department of Ophthalmology and Optometry, Fujian Medical University, Fuzhou , Fujian Province, China
Rong Lin Department of Ophthalmology and Optometry, Fujian Medical University, Fuzhou , Fujian Province, China
Ze-Ren Qiu The Third Clinical Medical College, Fujian Medical University, Fuzhou , Fujian Province, China
Ya-Min Chen Department of Ophthalmology and Optometry, Fujian Medical University, Fuzhou , Fujian Province, China
Ze-Qun Lin Department of Ophthalmology and Optometry, Fujian Medical University, Fuzhou , Fujian Province, China
Xiu-Bin Ke Department of Ophthalmology and Optometry, Fujian Medical University, Fuzhou , Fujian Province, China
Yan Huang Department of Ophthalmology and Optometry, Fujian Medical University, Fuzhou , Fujian Province, China; The Research Center for Juvenile Myopia Prevention and Control of Fujian Province, Fujian Medical University, Fuzhou , Fujian Province, China
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Abstract:
      AIM: To compare the damage of light-emitting diodes (LEDs) with different color rendering indexes (CRIs) to the ocular surface and retina of rats.

    METHODS: Totally 20 Sprague-Dawley (SD) rats were randomly divided into four groups: the first group was normal control group without any intervention, other three groups were exposed by LEDs with low (LED-L), medium (LED-M), and high (LED-H) CRI respectively for 12h a day, continuously for 4wk. The changes in tear secretion (Schirmer I test, SIt), tear film break-up time (BUT), and corneal fluorescein sodium staining (CFS) scores were compared at different times (1d before experiment, 2 and 4wk after the experiment). The histopathological changes of rat lacrimal gland and retina were observed at 4wk, and the expressions of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in lacrimal gland were detected by immunofluorescence method.

    RESULTS: With the increase of light exposed time, the CFS value of each light exposed group continued to increase, and the BUT and SIt scores continued to decrease, which were different from the control group, and the differences between the light exposed groups were statistically significant. Hematoxylin-eosin (HE) results showed that the lacrimal glands of each exposed group were seen varying degrees of acinar atrophy, vacuole distribution, increasing of eosinophil granules, etc.; the retina showed obvious reduction of photoreceptor cell layer and changes in retinal thickness; LED-L group has the most significant change in all tests. Immunofluorescence suggested that the positive expressions of TNF-α and IL-6 in the lacrimal glands of each exposed group were higher than those of the control group.

    CONCLUSION: LED exposure for 4wk can cause the pathological changes of lacrimal gland and retina of rats, and increase the expression of TNF-α and IL-6 in lacrimal gland, the degree of damage is negatively correlated with the CRI.

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