Citation:Xu Q,Wang Q,Hu LT,Lin J,Jiang N,Peng XD,Li C,Zhao GQ.NADPH oxidase 2 plays a protective role in experimental Aspergillus fumigatus keratitis in mice through killing fungi and limiting the degree of inflammation.Int J Ophthalmol 2022;15(7):1044-1052,doi:10.18240/ijo.2022.07.02
NADPH oxidase 2 plays a protective role in experimental Aspergillus fumigatus keratitis in mice through killing fungi and limiting the degree of inflammation
Received:May 26, 2021  Revised:March 28, 2022
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DOI:10.18240/ijo.2022.07.02
Key Words:NOX2  cornea  Aspergillus fumigatus  mice
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AuthorInstitution
Qiang Xu Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Qian Wang Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Li-Ting Hu Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Jing Lin Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Nan Jiang Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Xu-Dong Peng Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Cui Li Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Gui-Qiu Zhao Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
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Abstract:
      AIM: To explore whether nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2) is expressed in fungal keratitis in mice and investigate its role in this disease.

    METHODS: NOX2 expression was detected in C57BL/6 mice. After testing the inhibitory effect of diphenyleneiodonium chloride (DPI) on NOX2, its impact on clinical performance, myeloperoxidase levels, the number of colonies forming units, the level of H3, the generation of reactive oxygen species (ROS) and the release of cytokines [NF-κB, interleukin-17A (IL-17A), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), Nrf2, IL-10, and TGF-β] were compared. A one-way ANOVA and an unpaired, two-tailed Student’s t-test was used to determine the statistical significance.

    RESULTS: NOX2 expression was significantly increased after Aspergillus fumigatus injection in corneas and that this increase could be reduced by treatment with DPI. DPI treatment produced more severe inflammation and resulted in higher clinical scores, more neutrophils infiltration, a weakened ability to clear fungi, the release of fewer ROS and the formation of neutrophil extracellular traps. Treatment with DPI increased the expression of the proinflammatory cytokines NF-κB, IL-17A, IL-6, and TNF-α and decreased the expression of the anti-inflammatory cytokines Nrf2, IL-10 and TGF-β compared to their expression levels without DPI treatment.

    CONCLUSION: NOX2 plays an important role against Aspergillus fumigatus in the mouse cornea through killing fungi and limiting the degree of inflammation.

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