Citation:Shen J,Li Y,Li M,Liu WX,Sun HL,Zhang QP,Yi XN.Vascular endothelial growth factor-165b protects the blood-retinal barrier from damage after acute high intraocular pressure in rats.Int J Ophthalmol 2022;15(8):1231-1239,doi:10.18240/ijo.2022.08.02
Vascular endothelial growth factor-165b protects the blood-retinal barrier from damage after acute high intraocular pressure in rats
Received:May 25, 2021  Revised:May 27, 2022
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DOI:10.18240/ijo.2022.08.02
Key Words:vascular endothelial growth factor-165b  blood-retinal barrier  high intraocular pressure  Evans blue  zonula occludens-1
Fund Project:Supported by the National Natural Science Foundation of China (No.81660217); Youth Foundation of the First Affiliated Hospital of Hainan Medical University (No.HYYFYPY201922).
                    
AuthorInstitution
Jing Shen Department of Ophthalmology, First Affiliated Hospital of Hainan Medical University, Haikou , Hainan Province, China
Yi Li Grade 18 undergraduate of First Clinical College, Hainan Medical University, Haikou , Hainan Province, China
Min Li Department of Human Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha , Hunan Province, China
Wei-Xian Liu Department of Ophthalmology, First Affiliated Hospital of Hainan Medical University, Haikou , Hainan Province, China
Hong-Liang Sun Department of Ophthalmology, First Affiliated Hospital of Hainan Medical University, Haikou , Hainan Province, China
Quan-Peng Zhang Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou , Hainan Province, China; Anatomy Laboratory of Hainan Medical University, Haikou , Hainan Province, China
Xi-Nan Yi Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou , Hainan Province, China
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Abstract:
      AIM: To elucidate the role of vascular endothelial growth factor-165b (VEGF-165b) in blood-retinal barrier (BRB) injury in the rat acute glaucoma model.

    METHODS: In this study, the rat acute high intraocular pressure (HIOP) model was established before and after intravitreous injection of anti-VEGF-165b antibody. The expression of VEGF-165b and zonula occludens-1 (ZO-1) in rat retina was detected by double immunofluorescence staining and Western blotting, and the breakdown of BRB was detected by Evans blue (EB) dye.

    RESULTS: The intact retina of rats expressed VEGF-165b and ZO-1 protein, which were mainly located in the retinal ganglion cell layer and the inner nuclear layer and were both co-expressed with vascular endothelial cell markers CD31. After acute HIOP, the expression of VEGF-165b was up-regulated; the expression of ZO-1 was down-regulated at 12h and then recovered at 3d; EB leakage increased, peaking at 12h. After intravitreous injection of anti-VEGF-165b antibody, the expression of VEGF-165b protein was no significantly changed; and the down-regulation of the expression of ZO-1 was more obvious; EB leakage became more serious, peaking at 3d. EB analysis also showed that EB leakage in the peripheral retina was greater than that in the central retina.

    CONCLUSION: The endogenous VEGF-165b protein may protect the BRB from acute HIOP by regulating the expression of ZO-1. The differential destruction of BRB after acute HIOP may be related to the selective loss of retinal ganglion cells.

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