| Citation:Shao Y,Yu Y,Pei CG,Qu Y,Gao GP,Yang JL,Zhou Q,Yang L,Liu QP.The expression and distribution of α-Gal gene in various species ocular surface tissue.Int J Ophthalmol 2012;5(5):543-548,doi:10.3980/j.issn.2222-3959.2012.05.01 |
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| The expression and distribution of α-Gal gene in various species ocular surface tissue |
| Received:May 25, 2012 Revised:September 18, 2012 |
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| DOI：10.3980/j.issn.2222-3959.2012.05.01 |
| Key Words:α-Gal xenotransplantation animal ocular surface tissue engineering |
| Fund Project:National Natural Science Foundation of China (No. 81160118); Natural Science Foundation of Jiangxi Province (No. 20114BAB215029); Technology Foundation of Jiangxi Province (No. 20111BBG70026-2); Health Department Science and Technology Foundation (No.20121026); Education Department Youth Scientific Research Foundation (No.JJJ12158); National High Technology Research of China (863 project)(No. 2006AA02A131) |
| Author | Institution |
| Yi Shao |
Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
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| Yao Yu |
Department of Endocrinology, the Third Hospital of Nanchang, the Affiliated Nanchang Hospital of Southern Medical University, Nanchang , Jiangxi Province, China
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| Chong-Gang Pei |
Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
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| Yangluowa Qu |
Cullen Eye Institute of Baylor Universit, Houston, Texas 77030, USA |
| Gui-Ping Gao |
Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
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| Ji-Lin Yang |
Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
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| Qiong Zhou |
Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
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| Lu Yang |
Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
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| Qiu-Ping Liu |
Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
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| Abstract: |
| AIM: To examine the α-Gal gene expression and distribution in the different species/genus and developing phase animal ocular surface tissue. METHODS: α-Gal binding assay were carried out on various animal eye sections. Photograph, slit-lamp observation on various eye showed normal corneal transparence. RESULTS: A strong α-Gal expression in invertebrates and some vertebrates ocular tissue, but no α-Gal binding in birds, fish and mammal. α-Gal expression change in the development of mice ocular surface tissue (except sclera) and display genus dependency in the different murine ocular surface tissue. CONCLUSION: This study identified specific α-Gal epitopes binding area in the ocular surface of several species and may solve the problem that naive ocular surface may be used as natural α-Gal gene knockout model/high risk immunologic rejection model or ocular surface scaffold material. |
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