Citation:Shao Y,Yu Y,Pei CG,Qu Y,Gao GP,Yang JL,Zhou Q,Yang L,Liu QP.The expression and distribution of α-Gal gene in various species ocular surface tissue.Int J Ophthalmol 2012;5(5):543-548,doi:10.3980/j.issn.2222-3959.2012.05.01
The expression and distribution of α-Gal gene in various species ocular surface tissue
Received:May 25, 2012  Revised:September 18, 2012
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DOI:10.3980/j.issn.2222-3959.2012.05.01
Key Words:α-Gal  xenotransplantation  animal  ocular surface  tissue engineering
Fund Project:National Natural Science Foundation of China (No. 81160118); Natural Science Foundation of Jiangxi Province (No. 20114BAB215029); Technology Foundation of Jiangxi Province (No. 20111BBG70026-2); Health Department Science and Technology Foundation (No.20121026); Education Department Youth Scientific Research Foundation (No.JJJ12158); National High Technology Research of China (863 project)(No. 2006AA02A131)
                          
AuthorInstitution
Yi Shao Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
Yao Yu Department of Endocrinology, the Third Hospital of Nanchang, the Affiliated Nanchang Hospital of Southern Medical University, Nanchang , Jiangxi Province, China
Chong-Gang Pei Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
Yangluowa Qu Cullen Eye Institute of Baylor Universit, Houston, Texas 77030, USA
Gui-Ping Gao Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
Ji-Lin Yang Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
Qiong Zhou Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
Lu Yang Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
Qiu-Ping Liu Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang , Jiangxi Province, China
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Abstract:
      AIM: To examine the α-Gal gene expression and distribution in the different species/genus and developing phase animal ocular surface tissue.

    METHODS: α-Gal binding assay were carried out on various animal eye sections. Photograph, slit-lamp observation on various eye showed normal corneal transparence.

    RESULTS: A strong α-Gal expression in invertebrates and some vertebrates ocular tissue, but no α-Gal binding in birds, fish and mammal. α-Gal expression change in the development of mice ocular surface tissue (except sclera) and display genus dependency in the different murine ocular surface tissue.

    CONCLUSION: This study identified specific α-Gal epitopes binding area in the ocular surface of several species and may solve the problem that naive ocular surface may be used as natural α-Gal gene knockout model/high risk immunologic rejection model or ocular surface scaffold material.

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