Citation:Ren CJ,Zhou YF,Wu Y,Peng XD,Li C,Wang Q,Zhu GQ,You J,Zhang J,Zhao GQ,Lin J.Changes in corneal innervation and pain responses in fungal keratitis.Int J Ophthalmol 2020;13(1):1-6,doi:10.18240/ijo.2020.01.01
Changes in corneal innervation and pain responses in fungal keratitis
Received:July 30, 2019  Revised:November 20, 2019
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DOI:10.18240/ijo.2020.01.01
Key Words:keratitis  pain  fungal  innervation  subbasal nerve  mice
Fund Project:Supported by the National Natural Science Foundation of China (No.81470609; No.81870632); the Youth National Natural Science Foundation of China (No.81700800; No.81800800; No.81500695); Natural Science Foundation of Shandong Province (No.ZR2017MH008; No.ZR2017BH025).
                                
AuthorInstitution
Chang-Jie Ren Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
Yi-Fan Zhou Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
Yuan Wu Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
Xu-Dong Peng Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
Cui Li Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
Qian Wang Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
Guo-Qiang Zhu Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
Jia You Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
Jie Zhang Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
Gui-Qiu Zhao Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
Jing Lin Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 26600, Shandong Province, China
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Abstract:
      AIM: To characterize changes in the cornea nerve and pain responses in fungal keratitis (FK).

    METHODS: A retrospective analysis of in vivo confocal microscopy images of 11 FK corneas was performed, and the results were compared with those for 11 normal corneas. Subbasal corneal nerves were analyzed for total nerve number, main nerve trunk number, branching patterns and tortuosity. C57BL/6 mice were infected with Aspergillus fumigatus. Disease severity was determined through clinical scoring and slit lamp photography. Corneas were harvested at 1, 3, 5, and 7d post infection (p.i.) and assessed for β III tubulin. Corneal mechanical sensitivity thresholds were detected by von Frey test. β-endorphin (β-EP) and μ receptor protein expression was detected through Western blotting.

    RESULTS: Total nerve number, main nerve trunk number, and nerve branching were significantly lower in FK patients than in controls, but tortuosity was not significantly different. In infected mice, subbasal nerve density decreased from 1d p.i., reaching a minimum at 5d p.i. Clinical scores rose at 1d p.i., peaked at 3d p.i., and decreased at 5d p.i. Mechanical sensitivity thresholds showed the same trends. β-EP and μ receptor protein expression increased after infection.

    CONCLUSION: Corneal nerve density is lower in FK patients and Aspergillus fumigatus-infected mice than in controls. Pain sensitivity decreases with postinfection corneal ulcer aggravation. β-EP and μ receptor proteins are both upregulated in infected mouse corneas.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942951/
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