Citation:Wu YY,Zheng BR,Chen WZ,Guo MS,Huang YH,Zhang Y.Expression and role of autophagy related protein p62 and LC3 in the retina in a rat model of acute ocular hypertension.Int J Ophthalmol 2020;13(1):21-28,doi:10.18240/ijo.2020.01.04
Expression and role of autophagy related protein p62 and LC3 in the retina in a rat model of acute ocular hypertension
Received:July 10, 2019  Revised:October 22, 2019
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DOI:10.18240/ijo.2020.01.04
Key Words:glaucoma  acute ocular hypertension  LC3  p62  autophagy
Fund Project:Supported by the Natural Science Foundation of Fujian Province (No. 2016J01525).
                 
AuthorInstitution
Yu-Yu Wu Department of Ophthalmology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou , Fujian Province, China
Bing-Ru Zheng Department of Ophthalmology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou , Fujian Province, China
Wan-Zhu Chen Department of Ophthalmology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou , Fujian Province, China
Mao-Sheng Guo Department of Ophthalmology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou , Fujian Province, China
Yi-Hong Huang Department of Ophthalmology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou , Fujian Province, China
Yan Zhang Department of Ophthalmology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou , Fujian Province, China
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Abstract:
      AIM: To investigate the expression and possible role of the autophagy related protein p62 and LC3 in the retina based on a rat model of acute ocular hypertension.

    METHODS: Fifty rats were randomized into five groups: control group A, B, C, and D. Groups A to D all received normal saline perfusion into the anterior chamber with pressure of 80 mm Hg for one hour, and retina tissue was obtained at 6, 12, 24 and 48h after perfusion respectively, to investigate the activation of autophagy following ischemia-reperfusion. The distribution and semi-quantification of autophagy related protein p62 and LC3 in the retina were detected using immunohistochemistry technique. The expression level of these two proteins was evaluated using Western blot.

    RESULTS: The number of retinal ganglion cells (RGCs) decreased with increasing reperfusion time, and significant reduction in the retinal thickness was observed 48h after perfusion. In normal adult rats, LC3 protein was mainly expressed in the ganglion cell layer (GCL), and p62 protein was expressed in the nerve fiber layer (NFL), GCL, inner plexiform layer (IPL), inner nuclear layer (INL) and outer plexiform layer (OPL). In comparison to the control group, the expression level of LC3- II was higher in all the experimental groups (P<0.05), with the peak expression at 12h after reperfusion. Additionally, the expression level of p62 was higher in all the experimental groups than the control (P<0.05, except for group A), with the peak level occurred 24h after reperfusion.

    CONCLUSION: Both p62 and LC3 show low level and uneven expression in the retina of normal adult rats. Acute ocular hypertension can lead to upregulation of LC3- II and p62 expression in the retina. Autophagy flux is damaged 12h after reperfusion, potentially resulting in further loss of RGCs.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942946/
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