Citation:Zhao W,Dai L,Xi XT,Chen QB,An MX,Li Y.Sensitized heat shock protein 27 induces retinal ganglion cells apoptosis in rat glaucoma model.Int J Ophthalmol 2020;13(4):525-534,doi:10.18240/ijo.2020.04.01
Sensitized heat shock protein 27 induces retinal ganglion cells apoptosis in rat glaucoma model
Received:November 13, 2019  Revised:February 12, 2020
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DOI:10.18240/ijo.2020.04.01
Key Words:intraocular pressure  heat shock protein 27  retinal ganglion cells  autoimmune  apoptosis  glaucoma  rats
Fund Project:Supported by National Natural Science Foundation of China (No.81060077); Key Research and Development Program in Yunnan Province (International Science and Technology Cooperation, No.2017IB001); Science and Technology Planning Project of Guangzhou (No.201604020105).
                 
AuthorInstitution
Wei Zhao Department of Ophthalmology, the First Affiliated Hospital of Kunming Medical University, Kunming , Yunnan Province, China; Department of Ophthalmology, the First Affiliated Hospital of Dali University, Dali , Yunnan Province, China; Department of Ophthalmology, the Third Affiliated Hospital of Southern Medical University, Guangzhou , Guangdong Province, China
Le Dai Department of Ophthalmology, the First Affiliated Hospital of Kunming Medical University, Kunming , Yunnan Province, China
Xiao-Ting Xi Department of Ophthalmology, the First Affiliated Hospital of Kunming Medical University, Kunming , Yunnan Province, China
Qian-Bo Chen Department of Ophthalmology, the First Affiliated Hospital of Kunming Medical University, Kunming , Yunnan Province, China
Mei-Xia An Department of Ophthalmology, the Third Affiliated Hospital of Southern Medical University, Guangzhou , Guangdong Province, China
Yan Li Department of Ophthalmology, the First Affiliated Hospital of Kunming Medical University, Kunming , Yunnan Province, China
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Abstract:
      AIM: To investigate the relationships between the changes of heat shock protein 27 antibody (anti-HSP27) in serum/cerebrospinal fluid (CSF), intraocular pressure (IOP), retinal ganglion cell (RGC) apoptosis in a rat glaucoma model and disclose the underlying pathogenesis of glaucoma.

    METHODS: A total of 115 Wistar rats were randomly divided into 4 groups. Group 1 was the ocular hypertension group by condensing 3 episcleral & limbal veins or episcleral area of right eye (HP group, n=25) and sham operation group with conjunctiva incision without coagulation (n=25). Group 2: HSP27 or dose-matched PBS was injected into the vitreous (V-HSP27 group, n=15; V-PBS group, n=15). Group 3: HSP27 and complete Freund’s adjuvant or dose-matched PBS was injected subcutaneously into the hind limb accompanied intraperitoneal injection of pertussis toxin [sensitized group (I-HSP27 group), n=15; I-PBS group, n=15)]. Group 4 was normal group without any treatment (n=5). IOPs of the rats were measured before, day 3, weeks 1, 2, 4, 6, and 8 after treatment. Paraffin-embedded sections were prepared for HE staining and RGCs apoptosis were detected by TUNEL. Anti-HSP27 level in serum and CSF were examined by ELISA.

    RESULTS: IOPs were elevated significantly in HP and V-HSP27, V-PBS groups (P<0.01) and positively related to anti-HSP27 levels in serum and CSFs. Anti-HSP27 levels in serum and CSF were elevated significantly in I-HSP27 group compared to other groups (P<0.05). However, the IOPs did not show any relationship with the high-level anti-HSP27 in serum and CSFs. RGC apoptosis were all elevated significantly in the HP, V-HSP27, V-PBS and I-HSP27 groups and also positively relative with anti-HSP27 level in serum and CSFs except that high-level of anti-HSP27 in the serum of I-HSP group.

    CONCLUSION: The increases of anti-HSP27 levels in serum and CSFs both promote IOP escalation and the increase of RGC apoptosis in retina when anti-HSP27 is at low level. The case of high-level anti-HSP27 is opposite and shows protective function in preventing IOP increase and RGC apoptosis.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137695/
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