Citation:Tian R,Zou H,Wang LF,Song MJ,Liu L,Zhang H.Identification of microRNA-mRNA regulatory networks and pathways related to retinoblastoma across human and mouse.Int J Ophthalmol 2020;13(4):535-544,doi:10.18240/ijo.2020.04.02
Identification of microRNA-mRNA regulatory networks and pathways related to retinoblastoma across human and mouse
Received:July 20, 2019  Revised:February 19, 2020
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DOI:10.18240/ijo.2020.04.02
Key Words:Kyoto Encyclopedia of Genes and Genomes pathway  microRNA  retinoblastoma  weighted gene co-expression network analysis
Fund Project:Supported by the Youth Program of National natural science foundation (2018) of China (No.81802998); 2019 Basic Research Natural Science Foundation (No.20190201150JC); the Norman Bethune Program of Jilin University (No.2015327).
                 
AuthorInstitution
Rui Tian Department of Ophthalmology, the Second Hospital of Jilin University, Changchun , Jilin Province, China
He Zou Department of Ophthalmology, the Second Hospital of Jilin University, Changchun , Jilin Province, China
Lu-Fei Wang Department of Ophthalmology, the Second Hospital of Jilin University, Changchun , Jilin Province, China
Mei-Jiao Song Department of Ophthalmology, the Second Hospital of Jilin University, Changchun , Jilin Province, China
Lu Liu Department of Ophthalmology, the Second Hospital of Jilin University, Changchun , Jilin Province, China
Hui Zhang Department of Ophthalmology, the Second Hospital of Jilin University, Changchun , Jilin Province, China
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Abstract:
      AIM: To explore the mRNA and pathways related to retinoblastoma (RB) genesis and development.

    METHODS: Microarray datasets GSE29683 (human) and GSE29685 (mouse) were downloaded from NCBI GEO database. Homologous genes between the two species were identified using WGCNA, followed by protein-protein interaction (PPI) network construction and gene enrichment analysis. Disease-related miRNAs and pathways were retrieved from miR2Disease database and Comparative Toxicogenomics Database (CTD), respectively.

    RESULTS: A total of 352 homologous genes were identified. Two pathways including “cell cycle” and “pathway in cancer” in CTD and enrichment analysis were identified and seven miRNAs (including hsa-miR-373, hsa-miR-34a, hsa-miR-129, hsa-miR-494, hsa-miR-503, hsa-let-7 and hsa-miR-518c) were associated with RB. miRNAs modulate “cell cycle” and “pathway in cancer” pathways via regulating 13 genes (including CCND1, CDC25C, E2F2, CDKN2D and TGFB2).

    CONCLUSION: These results suggest that these miRNAs play crucial roles in RB genesis through “cell cycle” and “pathway in cancer” pathways by regulating their targets including CCND1, CDC25C, E2F2 and CDKN2D.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137714/
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