Citation:Deji QZ,Yan F,Zhaba WD,Liu YJ,Yin J,Huang ZP.Cross-talk between microRNA-let7c and transforming growth factor-β2 during epithelial-to-mesenchymal transition of retinal pigment epithelial cells.Int J Ophthalmol 2020;13(5):693-700,doi:10.18240/ijo.2020.05.01
Cross-talk between microRNA-let7c and transforming growth factor-β2 during epithelial-to-mesenchymal transition of retinal pigment epithelial cells
Received:February 09, 2020  Revised:March 17, 2020
Email this Article  Add to Favorites  Print
DOI:10.18240/ijo.2020.05.01
Key Words:microRNA-let7c  transforming growth factor-β2  epithelial-to-mesenchymal transition  human retinal pigment epithelial cells  nuclear factor-kappa B pathway
Fund Project:Supported by National Natural Science Foundation of China (No.81600754).
                 
AuthorInstitution
Qu-Zhen Deji Department of Ophthalmology, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing , Jiangsu Province, China
Feng Yan Department of Ophthalmology, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing , Jiangsu Province, China
Wang-Dui Zhaba Department of Neurosurgery, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing , Jiangsu Province, China
Ya-Jun Liu Department of Ophthalmology, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing , Jiangsu Province, China
Jie Yin Department of Ophthalmology, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing , Jiangsu Province, China
Zhen-Ping Huang Department of Ophthalmology, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing , Jiangsu Province, China
Hits: 335
Download times: 127
Abstract:
      AIM: To explore the roles of microRNA-let7c (miR-let7c) and transforming growth factor-β2 (TGF-β2) and cellular signaling during epithelial-to-mesenchymal transition (EMT) of retinal pigment epithelial cells.

    METHODS: Retinal pigment epithelial (ARPE-19) cells were cultured with no serum for 12h, and then with recombinant human TGF-β2 for different lengths of time. ARPE-19 cells were transfected with 1×106 TU/mL miR-let7c mimcs (miR-let7cM), miR-let7c mimcs negative control (miR-let7cMNC) and miR-let7c inhibitor (miR-let7cI) using the transfection reagent. The expression of keratin-18, vimentin, N-cadherin, IKB alpha, p65 were detected by Western blot, quantitative polymerase chain reaction and immunofluorescence.

    RESULTS: The expression of miR-let7c was dramatically reduced and the nuclear factor-kappa B (NF-κB) signaling pathway was activated after induction by TGF-β2 (P<0.05). In turn, overexpressed miR-let7c significantly inhibited TGF-β2-induced EMT (P<0.05). However, miR-let7c was unable to inhibit TGF-β2-induced EMT when the NF-κB signaling pathway was inhibited by BAY11-7082 (P<0.01).

    CONCLUSION: The miR-let7c regulates TGF-β2-induced EMT through the NF-κB signaling pathway in ARPE-19 cells.

PMC FullText Html:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201343/
Supplementary Material
PDF Fulltext  Download reader  HTML Fulltext   View/Add Comment