BASIC RESEARCH

Therapeutic effect of secretome from TNF-α stimulated mesenchymal stem cells in an experimental model of corneal limbal stem cell deficiency

Guo-Hu Di, Xia Qi, Jing Xu, Chao-Qun Yu, Qi-Long Cao, Zhi-Jun Xing, Zhi-Chao Li

This study aims to explore the secretome efficacy in tumor necrosis factor (TNF)-α stimulated mouse mesenchymal stem cells (MSCs) in a murine model of corneal limbal alkali injury. The therapeutic effects of topical application of concentrated conditioned medium from TNF-α stimulated MSCs (MSC-CMT) in an alkali-induced corneal limbal stem cell deficiency (LSCD) model were evaluated, and the possible underlying molecular mechanisms was studied. The study results revealed that MSC-CMT topical application alleviated the clinical symptoms of experimental LSCD, partially through TSG-6-dependent mechanisms.

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TNF-α刺激间充质干细胞分泌体对角膜缘干细胞缺乏症的治疗作用

该研究旨在探讨肿瘤坏死因子（TNF）-α刺激小鼠间充质干细胞（MSCs）产生的分泌体对角膜缘碱烧伤小鼠模型中的作用。研究人员将TNF-α刺激后的MSCs浓缩条件培养基（MSC-CMT）应用于碱烧伤角膜缘干细胞缺乏症模型，并探讨其可能的分子机制，研究结果表明，局部应用MSC-CMT可减轻实验性碱烧伤角膜缘干细胞缺乏症的临床症状，并一定程度上通过TSG-6依赖机制。

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BASIC RESEARCH

Effect of luteolin on apoptosis and vascular endothelial growth factor in human choroidal melanoma cells

Meng-Lin Shi, Yu-Fen Chen, Hong-Fei Liao

Traditional Chinese medicine has attracted much attention in recent years for its anti-tumor effects such as inducing tumor cell apoptosis and blocking cell cycle. This study investigated the effects of luteolin on the morphology, apoptosis, cell cycle, VEGF level of human choroidal melanoma cells C918 and OCM-1. Result showed that luteolin may induce apoptosis by regulating the levels of apoptosis related pathway proteins in C918 cells and OCM-1 cells. Luteolin can induce cell cycle arrest, decrease the expression of VEGF. The research provides the cell experimental basis for the application of luteolin in the treatment of human choroidal melanoma.

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木犀草素对人脉络膜黑色素瘤细胞凋亡及血管内皮生长因子的影响

近年来，中药因其诱导肿瘤细胞凋亡、阻断细胞周期等抗肿瘤作用而备受关注。该研究探讨木犀草素对人脉络膜黑色素瘤细胞C918和OCM-1的形态学、凋亡、细胞周期、VEGF水平的影响。结果表明，木犀草素可能通过调节C918和OCM-1细胞凋亡相关通路蛋白的水平来诱导细胞凋亡，诱导细胞周期阻滞，并降低VEGF的表达。该研究为木犀草素治疗人脉络膜黑色素瘤提供了细胞实验依据。

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CLINICAL RESEARCH

Surgical correction of recurrent epiblepharon in Chinese children using modified skin re-draping epicanthoplasty

Shou-Long Hu, Wen-Qing Shi, Ting Su, Qian-Min Ge, Qiu-Yu Li, Biao Li, Rong-Bin Liang, Pei-Wen Zhu, Yi Shao

The study aimed to evaluate the clinical efficacy of the modified skin re-draping epicanthoplasty procedure for correction of recurrent lower lid epiblepharon in Chinese children. Congenital epiblepharon is one of the common eyelid anomalies among orient children. The researchers describe a surgical technique to correct recurrent epiblepharon and its clinical results. The study confirmed that this surgical modified skin re-draping technique is effective and highly satisfactory for correction of recurrent epiblepharon. The approach is characterized by a simple design, a straightforward procedure, inconspicuous scarring, and good postoperative appearance.

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改良的皮肤悬吊内眦赘皮矫正术治疗中国儿童复发性内眦赘皮

该研究旨在探讨改良的皮肤悬吊内眦赘皮矫正术治疗中国儿童复发性下睑型内眦赘皮的临床疗效。先天性内眦赘皮是东方儿童常见的眼睑畸形之一。作者描述了一种新的手术技术矫正复发性内眦赘皮及其临床结果。研究结果证实改良的皮肤悬吊技术用于矫正复发性内眦赘皮效果良好，并获得较好的满意度。这个方法设计精简，操作简单，疤痕不明显，术后外观良好。

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CLINICAL RESEARCH

Analysis of the etiologies, treatments and prognoses in children and adolescent vitreous hemorrhage

Zheng-Wei Liu, Jie Peng, Chun-Li Chen, Xue-Hao Cui, Pei-Quan Zhao

Vitreous hemorrhage (VH) was one of the serious blinding diseases. The etiology is complicated and diverse. The incidence rate of vitreous hemorrhage in children was much higher than that of adults, but previous research was often rarely. Totally 262 eyes of 210 cases (≤18y) with VH was collected to determine the etiologies, treatment modalities and visual outcomes. Compared with previous studies, the mean age at diagnosis (1y) in this study was the youngest one; the sum of spontaneous vitreous hemorrhage was the largest one; all patients were also divided into 6 subgroups, in accordance to physiological and psychological development rather than equidistant age. Retinopathy of prematurity was the primary cause of VH in children and adolescent. In contrast with spontaneous VH, visual acuity was obvious improved after appropriate treatment in traumatic VH. Initial visual acuity was most important predictor for visual outcome.

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儿童和青少年玻璃体出血的病因分析、治疗及预后

玻璃体出血（VH）是严重致盲性疾病之一。病因复杂多样。儿童玻璃体出血的发生率远高于成人，但以往的研究很少。该研究收集210例（262眼，18岁以下）玻璃体出血儿童，分析其病因、治疗方法及预后视力。与以往研究相比，该研究的平均诊断年龄最小(1y)，自发性玻璃体出血病例最多；根据生理和心理发展而非等距年龄将所有患者分为6个亚组。儿童和青少年玻璃体出血最常见的病因是早产儿视网膜病变。外伤性玻璃体积血经适当治疗后视力明显改善，而自发性玻璃体积血改善不明显。初始视力是视力预后最重要的预测指标。

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