AIM:To investigate the clinical effect of mitomycin C(MMC)combined with adjustable suture used in refractory glaucoma.METHODS:A total of 39 patients 48 eyes with refractory glaucoma were treated by MMC combined with adjustable sutures,postoperative anterior chamber,intraocular pressure(IOP),bleb and complications were followed up for 6-24 months with an average of 15 months.RESULTS:The IOP had good control in surgery,success rate was 94% and functional blebs was 94%;complications were mainly shallow anterior chamber,up to 6%.CONCLUSION:The use of filtering surgery with MMC and the adjustable sutures is effective in preventing fibrosis of bleb in various types of refractory glaucoma with well controlled IOP,and it can greatly improve the success rate of surgery,and should be widely applied.